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验证数据展示

  • WB analysis of human liver using 11843-1-AP

    WB analysis of human liver using 11843-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 11843-1-AP (RBP5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 11843-1-AP

    IHC staining of human liver cancer using 11843-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 11843-1-AP (RBP5 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver cancer using 11843-1-AP

    IHC staining of human liver cancer using 11843-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 11843-1-AP (RBP5 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 11843-1-AP

    IF Staining of HepG2 using 11843-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RBP5 antibody (11843-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

RBP5 Polyclonal antibody

RBP5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11843-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

HRBPiso, CRBP-III, CRBPIII, CRBP3, CRBP III

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inhuman liver tissue, pig liver tissue, rat liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11843-1-AP targets RBP5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, rat, pig
Immunogen RBP5 fusion protein Ag2485 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name retinol binding protein 5, cellular
Synonyms HRBPiso, CRBP-III, CRBPIII, CRBP3, CRBP III
Calculated Molecular Weight 135 aa, 16 kDa
Observed Molecular Weight 16-18 kDa
GenBank Accession NumberBC029355
Gene Symbol RBP5
Gene ID (NCBI) 83758
RRIDAB_2085321
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP82980
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RBP5 antibody 11843-1-APDownload protocol
IHC protocol for RBP5 antibody 11843-1-APDownload protocol
IF protocol for RBP5 antibody 11843-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols