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验证数据展示

  • WB analysis of HEK-293 using 14541-1-AP

    WB analysis of HEK-293 using 14541-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14541-1-AP (RBP7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of human kidney using 14541-1-AP

    IF Staining of human kidney using 14541-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using RBP7 antibody (14541-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IP experiment of HEK-293 using 14541-1-AP

    IP experiment of HEK-293 using 14541-1-AP

    IP result of anti-RBP7 (IP:14541-1-AP, 4ug; Detection:14541-1-AP 1:500) with HEK-293 cells lysate 3000ug.

  • WB analysis of mouse adipose using 14541-1-AP

    WB analysis of mouse adipose using 14541-1-AP

    mouse adipose tissue were subjected to SDS PAGE followed by western blot with 14541-1-AP (RBP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 14541-1-AP

    IHC staining of human colon cancer using 14541-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14541-1-AP (RBP7 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 14541-1-AP

    IF Staining of HEK-293 using 14541-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using RBP7 antibody (14541-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

RBP7 Polyclonal antibody

RBP7 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Cat No. 14541-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IF-P, IP, ELISA

别名

CRBP4, CRABP-IV, CRABP4, CRABP IV, Cellular retinoic acid-binding protein IV

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse adipose tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman kidney tissue, human colon cancer tissue
Positive IF/ICC detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14541-1-AP targets RBP7 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原 RBP7 fusion protein Ag6045 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 retinol binding protein 7, cellular
别名 CRBP4, CRABP-IV, CRABP4, CRABP IV, Cellular retinoic acid-binding protein IV
计算分子量 15.5 kDa
观测分子量 15-18 kDa
GenBank蛋白编号BC063013
基因名称 RBP7
Gene ID (NCBI) 116362
RRIDAB_2179293
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96R05
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RBP7 antibody 14541-1-APDownload protocol
IHC protocol for RBP7 antibody 14541-1-APDownload protocol
IF protocol for RBP7 antibody 14541-1-APDownload protocol
IP protocol for RBP7 antibody 14541-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Cell Int

RBP7 functions as a tumor suppressor in HR + breast cancer by inhibiting the AKT/SREBP1 pathway and reducing fatty acid

Authors - Yue Yu
View Article