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RBP7 Polyclonal antibody

RBP7 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

Cat no : 14541-1-AP

Print datasheet

Synonyms

CRBP4, CRABP-IV, CRABP4, CRABP IV, Cellular retinoic acid-binding protein IV

验证数据展示

  • WB analysis of HEK-293 using 14541-1-AP

    WB analysis of HEK-293 using 14541-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14541-1-AP (RBP7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 14541-1-AP

    IHC staining of human colon cancer using 14541-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 14541-1-AP (RBP7 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon cancer using 14541-1-AP

    IHC staining of human colon cancer using 14541-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 14541-1-AP (RBP7 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of human kidney using 14541-1-AP

    IF Staining of human kidney using 14541-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using RBP7 antibody (14541-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IP experiment of HEK-293 using 14541-1-AP

    IP experiment of HEK-293 using 14541-1-AP

    IP result of anti-RBP7 (IP:14541-1-AP, 4ug; Detection:14541-1-AP 1:500) with HEK-293 cells lysate 3000ug.

  • IF Staining of HEK-293 using 14541-1-AP

    IF Staining of HEK-293 using 14541-1-AP

    Immunofluorescent analysis of HEK-293 cells using 14541-1-AP (RBP7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis of mouse adipose using 14541-1-AP

    WB analysis of mouse adipose using 14541-1-AP

    mouse adipose tissue were subjected to SDS PAGE followed by western blot with 14541-1-AP (RBP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, mouse adipose tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman kidney tissue, human colon cancer tissue
Positive IF/ICC detected inHEK-293 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14541-1-AP targets RBP7 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen RBP7 fusion protein Ag6045 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name retinol binding protein 7, cellular
Synonyms CRBP4, CRABP-IV, CRABP4, CRABP IV, Cellular retinoic acid-binding protein IV
Calculated Molecular Weight 15.5 kDa
Observed Molecular Weight 15-18 kDa
GenBank Accession NumberBC063013
Gene Symbol RBP7
Gene ID (NCBI) 116362
RRIDAB_2179293
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96R05
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RBP7 antibody 14541-1-APDownload protocol
IHC protocol for RBP7 antibody 14541-1-APDownload protocol
IF protocol for RBP7 antibody 14541-1-APDownload protocol
IP protocol for RBP7 antibody 14541-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Cell Int

RBP7 functions as a tumor suppressor in HR + breast cancer by inhibiting the AKT/SREBP1 pathway and reducing fatty acid

Authors - Yue Yu
View Article