RBPJ Polyclonal antibody
RBPJ Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, MCF-7 cells, NIH/3T3 cells |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
30044-1-AP targets RBPJ in WB, IP, IHC, ELISA applications and shows reactivity with Human, mouse samples.
Tested Applications | WB, IP, IHC, ELISA Application Description |
Tested Reactivity | Human, mouse |
Immunogen | RBPJ fusion protein Ag32552 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | recombination signal binding protein for immunoglobulin kappa J region |
Synonyms | CBF 1, CBF1, csl, IGKJRB, IGKJRB1, KBF2, RBP J, RBP J kappa, RBP JK, RBPJ, RBPJK, RBPSUH, SUH |
Calculated Molecular Weight | 56 kDa |
Observed Molecular Weight | ~60 kDa |
GenBank Accession Number | BC064976 |
Gene Symbol | RBPJ |
Gene ID (NCBI) | 3516 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q06330 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for RBPJ antibody 30044-1-AP | Download protocol |
IHC protocol for RBPJ antibody 30044-1-AP | Download protocol |
IP protocol for RBPJ antibody 30044-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |