验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, MCF-7 cells, NIH/3T3 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IP | See 2 publications below |
产品信息
30044-1-AP targets RBPJ in WB, IHC, IP, ELISA applications and shows reactivity with Human, mouse samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | IP |
| 经测试反应性 | Human, mouse |
| 文献引用反应性 | human |
| 免疫原 | RBPJ fusion protein Ag32552 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | recombination signal binding protein for immunoglobulin kappa J region |
| 别名 | CBF 1, CBF1, csl, IGKJRB, IGKJRB1, KBF2, RBP J, RBP J kappa, RBP JK, RBPJ, RBPJK, RBPSUH, SUH |
| 计算分子量 | 56 kDa |
| 观测分子量 | ~60 kDa |
| GenBank蛋白编号 | BC064976 |
| 基因名称 | RBPJ |
| Gene ID (NCBI) | 3516 |
| RRID | AB_3086217 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q06330 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RBPJ, also named as IGKJRB, IGKJRB1, RBPJK, RBPSUH, NY-REN-30, CBF-1 and CSL, belongs to the Su(H) family. It is a transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. RBPJ acts as a transcriptional repressor when it is not associated with Notch proteins. It is specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. RBPJ binds to the Runx3 promoter in the atrioventricular canal in vivo, and inhibition of Notch reduces RUNX3 expression in the cardiac cushion of embryonic hearts.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RBPJ antibody 30044-1-AP | Download protocol |
| IHC protocol for RBPJ antibody 30044-1-AP | Download protocol |
| IP protocol for RBPJ antibody 30044-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO Mol Med Reciprocal inhibition of NOTCH and SOX2 shapes tumor cell plasticity and therapeutic escape in triple-negative breast cancer |
