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验证数据展示

  • WB analysis of HepG2 using 30044-1-AP

    WB analysis of HepG2 using 30044-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 30044-1-AP (RBPJ antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 30044-1-AP

    IP experiment of HepG2 using 30044-1-AP

    IP result of anti-RBPJ (IP:30044-1-AP, 4ug; Detection:30044-1-AP 1:1000) with HepG2 cells lysate 1080 ug.

  • IHC staining of human pancreas cancer using 30044-1-AP

    IHC staining of human pancreas cancer using 30044-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 30044-1-AP (RBPJ antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RBPJ antibody included

RBPJ Polyclonal antibody

RBPJ Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 30044-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IP, IHC, ELISA

Synonyms

CBF 1, CBF1, csl, IGKJRB, IGKJRB1, KBF2, RBP J, RBP J kappa, RBP JK, RBPJ, RBPJK, RBPSUH, SUH

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, MCF-7 cells, NIH/3T3 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RBPJ Ready-To-Use IHC Kit. RBPJ primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IPSee 1 publications below

产品信息

30044-1-AP targets RBPJ in WB, IHC, IP, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsIP
Tested Reactivity Human, mouse
Cited Reactivityhuman
Immunogen RBPJ fusion protein Ag32552 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name recombination signal binding protein for immunoglobulin kappa J region
Synonyms CBF 1, CBF1, csl, IGKJRB, IGKJRB1, KBF2, RBP J, RBP J kappa, RBP JK, RBPJ, RBPJK, RBPSUH, SUH
Calculated Molecular Weight 56 kDa
Observed Molecular Weight~60 kDa
GenBank Accession NumberBC064976
Gene Symbol RBPJ
Gene ID (NCBI) 3516
RRIDAB_3086217
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ06330
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RBPJ, also named as IGKJRB, IGKJRB1, RBPJK, RBPSUH, NY-REN-30, CBF-1 and CSL, belongs to the Su(H) family. It is a transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. RBPJ acts as a transcriptional repressor when it is not associated with Notch proteins. It is specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. RBPJ binds to the Runx3 promoter in the atrioventricular canal in vivo, and inhibition of Notch reduces RUNX3 expression in the cardiac cushion of embryonic hearts.

实验方案

Product Specific Protocols
WB protocol for RBPJ antibody 30044-1-APDownload protocol
IHC protocol for RBPJ antibody 30044-1-APDownload protocol
IP protocol for RBPJ antibody 30044-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIP

EMBO Mol Med

Reciprocal inhibition of NOTCH and SOX2 shapes tumor cell plasticity and therapeutic escape in triple-negative breast cancer

Authors - Morgane Fournier
View Article