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RBX1 Monoclonal antibody, PBS Only

RBX1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F4D9

Cat no : 66716-1-PBS

Print datasheet

Synonyms

Protein ZYP, EC:2.3.2.27, E3 ubiquitin-protein transferase RBX1, E3 ubiquitin-protein ligase RBX1, N-terminally processed, E3 ubiquitin-protein ligase RBX1

验证数据展示

  • IHC staining of human liver cancer using 66716-1-Ig (same clone as 66716-1-PBS)

    IHC staining of human liver cancer using 66716-1-Ig (same clone as 66716-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66716-1-Ig (RBX1 antibody) at dilution of 1:5000 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 66716-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RBX1 antibody included
  • IHC staining of human liver cancer using 66716-1-Ig (same clone as 66716-1-PBS)

    IHC staining of human liver cancer using 66716-1-Ig (same clone as 66716-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66716-1-Ig (RBX1 antibody) at dilution of 1:5000 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 66716-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RBX1 antibody included
  • WB analysis using 66716-1-Ig (same clone as 66716-1-PBS)

    WB analysis using 66716-1-Ig (same clone as 66716-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66716-1-Ig (RBX1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66716-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 66716-1-Ig (same clone as 66716-1-PBS)

    IF Staining of A431 using 66716-1-Ig (same clone as 66716-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RBX1 antibody (66716-1-Ig, Clone: 1F4D9 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 66716-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy RBX1 Ready-To-Use IHC Kit. RBX1 primary antibody included.

产品信息

66716-1-PBS targets RBX1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RBX1 fusion protein Ag7005 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ring-box 1
Synonyms Protein ZYP, EC:2.3.2.27, E3 ubiquitin-protein transferase RBX1, E3 ubiquitin-protein ligase RBX1, N-terminally processed, E3 ubiquitin-protein ligase RBX1
Calculated Molecular Weight 12 kDa
Observed Molecular Weight 12-15 kDa
GenBank Accession NumberBC001466
Gene Symbol RBX1
Gene ID (NCBI) 9978
RRIDAB_2882067
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP62877
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RBX1(RING-box protein 1) is also named as RNF75, ROC1 and is a requisite component of the multi- subunit SCF family E3s36-40. It mediates the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair.