• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 31234-1-AP

    WB analysis using 31234-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31234-1-AP (RC3H1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 31234-1-AP

    IHC staining of human ovary cancer using 31234-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31234-1-AP (RC3H1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 31234-1-AP

    IF Staining of HeLa using 31234-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RC3H1 antibody (31234-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

RC3H1 Polyclonal antibody

RC3H1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31234-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

ROQUIN, RNF198, RING finger protein 198, KIAA2025

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected in4T1 cells, HEK-293T cells, HeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31234-1-AP targets RC3H1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 RC3H1 fusion protein Ag35089 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ring finger and CCCH-type zinc finger domains 1
别名 ROQUIN, RNF198, RING finger protein 198, KIAA2025
计算分子量126 kDa
观测分子量130-150 kDa
GenBank蛋白编号NM_001300850
基因名称 RC3H1
Gene ID (NCBI) 149041
RRIDAB_3669912
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ5TC82
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Roquin-1 (Rc3h1) is an E3 ubiquitin ligase originally discovered in a mutational screen for genetic factors contributory to systemic lupus erythematosus-like symptoms in mice. A single base pair mutation in the Rc3h1 gene resulted in the manifestation of autoantibody production and sustained immunological inflammation characterized by excessive T follicular helper cell activation and formation of germinal centers. (PMID: 26673963)

实验方案

Product Specific Protocols
WB protocol for RC3H1 antibody 31234-1-APDownload protocol
IHC protocol for RC3H1 antibody 31234-1-APDownload protocol
IF protocol for RC3H1 antibody 31234-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols