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RCAS1 Monoclonal antibody, PBS Only

RCAS1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4H8A12

Cat No : 66170-1-PBS

Print datasheet

Synonyms

EB9, EBAG9, PDAF, RCAS1

验证数据展示

  • IHC staining of human breast cancer using 66170-1-Ig (same clone as 66170-1-PBS)

    IHC staining of human breast cancer using 66170-1-Ig (same clone as 66170-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66170-1-Ig (RCAS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66170-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RCAS1 antibody included
  • IHC staining of human breast cancer using 66170-1-Ig (same clone as 66170-1-PBS)

    IHC staining of human breast cancer using 66170-1-Ig (same clone as 66170-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66170-1-Ig (RCAS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66170-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RCAS1 antibody included
  • WB analysis of HEK-293 using 66170-1-Ig (same clone as 66170-1-PBS)

    WB analysis of HEK-293 using 66170-1-Ig (same clone as 66170-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66170-1-Ig (RCAS1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66170-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 66170-1-Ig (same clone as 66170-1-PBS)

    IF Staining of MCF-7 using 66170-1-Ig (same clone as 66170-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RCAS1 antibody (66170-1-Ig, Clone: 4H8A12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66170-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy RCAS1 Ready-To-Use IHC Kit. RCAS1 primary antibody included.

产品信息

66170-1-PBS targets RCAS1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RCAS1 fusion protein Ag2905 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name estrogen receptor binding site associated, antigen, 9
Synonyms EB9, EBAG9, PDAF, RCAS1
Calculated Molecular Weight 213 aa, 24 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC017729
Gene Symbol EBAG9
Gene ID (NCBI) 9166
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO00559
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Estrogen receptor-binding fragment-associated antigen 9 (EBAG9) gene was identified as an estrogen-responsive gene. The gene product, receptor-binding cancer antigen expressed on SiSo cells (RCAS1), is associated with aggressive characteristics and poor overall survival for 15 different human malignancies. The correlation between RCAS1 expression and several clinicopathological variables, including tumor size, clinical stage, invasion depth and lymph node metastasis highlights this molecule's clinical significance. Expression of RCAS1 in tumor cells plays an important role in evasion from host immune system resulting tumor progression, invasion and metastasis. Further exploration of RCAS1 biological function will facilitate development of novel therapeutic strategies that target RCAS1.