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RCL1 Polyclonal antibody

RCL1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 15330-1-AP

Print datasheet

Synonyms

RTC2, RPC2, RNAC, RNA 3'-terminal phosphate cyclase-like protein, HSPC338

验证数据展示

  • WB analysis of A431 using 15330-1-AP

    WB analysis of A431 using 15330-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 15330-1-AP (RCL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of A375 using 15330-1-AP

    IF Staining of A375 using 15330-1-AP

    Immunofluorescent analysis of A375 cells using 15330-1-AP (RCL1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA431 cells, HepG2 cells, A375 cells, MCF-7 cells
Positive IF/ICC detected inA375 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15330-1-AP targets RCL1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen RCL1 fusion protein Ag7058 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RNA terminal phosphate cyclase-like 1
Synonyms RTC2, RPC2, RNAC, RNA 3'-terminal phosphate cyclase-like protein, HSPC338
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC001025
Gene Symbol RCL1
Gene ID (NCBI) 10171
RRIDAB_2180010
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y2P8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RCL1 antibody 15330-1-APDownload protocol
IF protocol for RCL1 antibody 15330-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

J Mol Cell Biol

Stability and function of RCL1 are dependent on interaction with BMS1

Authors - Yong Wang
View Article
humanWB,IF

Cancer Cell Int

Rcl1 suppresses tumor progression of hepatocellular carcinoma: a comprehensive analysis of bioinformatics and in vitro experiments.

Authors - Yu Jiaze
View Article