验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68221-1-Ig targets RCL1 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, Mouse, Rat |
| 免疫原 | RCL1 fusion protein Ag7058 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | RNA terminal phosphate cyclase-like 1 |
| 别名 | RCL1, RNAC, RPC2, RPCL1, RTC2 |
| 计算分子量 | 41 kDa |
| 观测分子量 | 41 kDa |
| GenBank蛋白编号 | BC001025 |
| 基因名称 | RCL1 |
| Gene ID (NCBI) | 10171 |
| RRID | AB_2935309 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9Y2P8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RNA 3'-terminal phosphate cyclase-like protein (RCL1) does not have cyclase activity. It plays a role in 40S-ribosomal-subunit biogenesis in the early pre-rRNA processing steps at sites A0, A1 and A2 that are required for proper maturation of the 18S RNA. RCL1 has two isoforms with the molecular weight of 41 kDa and 20 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RCL1 antibody 68221-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |