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RCL1 Monoclonal antibody

RCL1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D1B3

Cat no : 68221-1-Ig

Print datasheet

Synonyms

RCL1, RNAC, RPC2, RPCL1, RTC2

验证数据展示

  • WB analysis using 68221-1-Ig

    WB analysis using 68221-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68221-1-Ig (RCL1 antibody) at dilution of 1:20000 and incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

经过测试的应用

Positive WB detected inMCF-7 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68221-1-Ig targets RCL1 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen RCL1 fusion protein Ag7058 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name RNA terminal phosphate cyclase-like 1
Synonyms RCL1, RNAC, RPC2, RPCL1, RTC2
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC001025
Gene Symbol RCL1
Gene ID (NCBI) 10171
RRIDAB_2935309
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y2P8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNA 3'-terminal phosphate cyclase-like protein (RCL1) does not have cyclase activity. It plays a role in 40S-ribosomal-subunit biogenesis in the early pre-rRNA processing steps at sites A0, A1 and A2 that are required for proper maturation of the 18S RNA. RCL1 has two isoforms with the molecular weight of 41 kDa and 20 kDa.

实验方案

Product Specific Protocols
WB protocol for RCL1 antibody 68221-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols