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RCOR1 Polyclonal antibody
RCOR1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | C2C12 cell, PC-12 cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IHC | See 1 publications below |
产品信息
27686-1-AP targets RCOR1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Immunogen | RCOR1 fusion protein Ag26770 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | REST corepressor 1 |
| Synonyms | RCOR 1, RCOR, COREST |
| Calculated Molecular Weight | 53 kDa |
| Observed Molecular Weight | 60-65 kDa |
| GenBank Accession Number | NM_015156 |
| Gene Symbol | RCOR1 |
| Gene ID (NCBI) | 23186 |
| RRID | AB_2880947 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RCOR1, also named as REST corepressor 1, is a 485 amino acid protein, which belongs to the CoREST family. RCOR1 is a essential component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. The calculated molecular weight of RCOR1 is a 53 kDa, but the phosphorylated RCOR1 is about 60-65 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RCOR1 antibody 27686-1-AP | Download protocol |
| IHC protocol for RCOR1 antibody 27686-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biochem Biophys Res Commun Increased NRSF/REST in anterior cingulate cortex contributes to diabetes-related neuropathic pain. | ||
Ann Diagn Pathol microRNA 155 up regulation in the CNS is strongly correlated to Down's syndrome dementia. | ||
Nat Commun Demethylase-independent roles of LSD1 in regulating enhancers and cell fate transition
| ||
Cell Chem Biol HDAC3 and HDAC8 PROTAC dual degrader reveals roles of histone acetylation in gene regulation | ||
Cell Rep E3 ligase Trim35 inhibits LSD1 demethylase activity through K63-linked ubiquitination and enhances anti-tumor immunity in NSCLC |
