验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HEK-293 cells |
| Positive IHC detected in | mouse embryo tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 4 publications below |
| CoIP | See 1 publications below |
产品信息
23969-1-AP targets RCOR2 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | RCOR2 fusion protein Ag21112 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | REST corepressor 2 |
| 别名 | CoREST2, RCOR2, REST corepressor 2 |
| 计算分子量 | 523 aa, 58 kDa |
| 观测分子量 | 58 kDa |
| GenBank蛋白编号 | BC023587 |
| 基因名称 | RCOR2 |
| Gene ID (NCBI) | 283248 |
| RRID | AB_2879382 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | Q8IZ40 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
In mammals, the CoREST (corepressor for element-1-silencing transcription factor) complex is a chromatin-modifying structure that, through interactions with NRSF (neuron restrictive silencer factor), regulates neuronal gene expression and neuronal cell fate. RCOR2 (REST corepressor 2) and RCOR3 (REST corepressor 3) are nuclear proteins that each contain one ELM2 domain and two SANT domains. RCOR2 and RCOR3, both members of the CoREST family, are thought to function as components of the CoREST complex, possibly playing a role in the transcriptional repression of neuronal genes. Additionally, RCOR2 and RCOR3, in conjunction with CoREST, can form immunocomplexes with a variety of histone-modifying genes, including G9a and HDAC1. Via these protein complexes, RCOR2 and RCOR3 can further regulate transcription by controlling the methylation and demethylation of target genes during early development.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RCOR2 antibody 23969-1-AP | Download protocol |
| IHC protocol for RCOR2 antibody 23969-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Merkel cell polyomavirus activates LSD1-mediated blockade of non-canonical BAF to regulate transformation and tumorigenesis. | ||
Nat Commun The scaffolding function of LSD1 controls DNA methylation in mouse ESCs
| ||
Nat Commun Demethylase-independent roles of LSD1 in regulating enhancers and cell fate transition
| ||
Int Immunopharmacol Identification and validation of the prognostic signature of a novel demethylation-related gene associated with the clinical features of colon cancer
|
