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RD3 Polyclonal antibody

RD3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14855-1-AP

Print datasheet

Synonyms

C1orf36, LCA12, Protein RD3, RD3, retinal degeneration 3

验证数据展示

  • WB analysis using 14855-1-AP

    WB analysis using 14855-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14855-1-AP (RD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of rat eye using 14855-1-AP

    IHC staining of rat eye using 14855-1-AP

    Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 14855-1-AP (RD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat eye using 14855-1-AP

    IHC staining of rat eye using 14855-1-AP

    Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 14855-1-AP (RD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse retina tissue, rat retina tissue
Positive IHC detected inrat eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14855-1-AP targets RD3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RD3 fusion protein Ag6641 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name retinal degeneration 3
Synonyms C1orf36, LCA12, Protein RD3, RD3, retinal degeneration 3
Calculated Molecular Weight22.7 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC065541
Gene Symbol RD3
Gene ID (NCBI) 343035
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z3Z2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RD3, also named as C1orf36, is a part of subnuclear protein complexes involved in diverse processes, such as transcription and splicing. It is associated with Retinal Degeneration.

实验方案

Product Specific Protocols
WB protocol for RD3 antibody 14855-1-APDownload protocol
IHC protocol for RD3 antibody 14855-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols