验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, A549 cells, mouse liver tissue, mouse kidney tissue, rat kidney tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy RDH10 Ready-To-Use IHC Kit. RDH10 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14644-1-AP targets RDH10 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, insect |
| 免疫原 | RDH10 fusion protein Ag6237 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | retinol dehydrogenase 10 (all-trans) |
| 别名 | RDH10, Retinol dehydrogenase 10, SDR16C4 |
| 计算分子量 | 38 kDa |
| 观测分子量 | 39 kDa |
| GenBank蛋白编号 | BC067131 |
| 基因名称 | RDH10 |
| Gene ID (NCBI) | 157506 |
| RRID | AB_2177866 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IZV5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RDH10(Retinol dehydrogenase 10) is a strictly NAD+-dependent enzyme with multisubstrate specificity that recognizes cis-retinols as well as all-trans-retinol as substrates. RDH10-mediated oxidation of retinol plays as important a role in the control and regulation of RA production during embryogenesis as does the subsequent RALDH-mediated reaction(PMID: 22319578).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RDH10 antibody 14644-1-AP | Download protocol |
| IHC protocol for RDH10 antibody 14644-1-AP | Download protocol |
| IP protocol for RDH10 antibody 14644-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Generation of a ciliary margin-like stem cell niche from self-organizing human retinal tissue. | ||
Proc Natl Acad Sci U S A Retinoic acid signaling determines the fate of uterine stroma in the mouse Müllerian duct. | ||
Cell Prolif Omega-3 polyunsaturated fatty acids and its metabolite 12-HEPE rescue busulfan disrupted spermatogenesis via target to GPR120 | ||
Stem Cell Reports Intercellular Adhesion-Dependent Cell Survival and ROCK-Regulated Actomyosin-Driven Forces Mediate Self-Formation of a Retinal Organoid. |
