验证数据展示
经过测试的应用
| Positive WB detected in | mouse eye tissue, rat eye tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13140-1-AP targets RDH5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | RDH5 fusion protein Ag3784 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | retinol dehydrogenase 5 (11-cis/9-cis) |
| 别名 | 11 cis RDH, 11 cis retinol dehydrogenase, HSD17B9, RDH1, RDH5, SDR9C5 |
| 计算分子量 | 318 aa, 35 kDa |
| 观测分子量 | 32 kDa |
| GenBank蛋白编号 | BC028298 |
| 基因名称 | RDH5 |
| Gene ID (NCBI) | 5959 |
| RRID | AB_2918031 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92781 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RDH5, also named as RDH1, SDR9C5 and HSD17B9, belongs to short-chain dehydrogenases/reductases (SDR) family. RDH5 catalyzes the oxidation of cis-isomers of retinol in an NAD-dependent manner for the final step in the biosynthesis of 11-cis retinaldehyde. Mutations in this gene are associated with fundus albipunctatus, an autosomal recessive eye disease characterized by stationary night blindness and accumulation of white spots in the retina (PubMed:11675386). The predicted size of this protein is 35 kDa. Some paper have reported that the molecular weight is 32 kDa and could form a dimer of about 60 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RDH5 antibody 13140-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |