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RDM1 Monoclonal antibody, PBS Only

RDM1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C10B4

Cat no : 66941-1-PBS

Print datasheet

Synonyms

RAD52 homolog B, RAD52 motif 1, RAD52B, RDM1

验证数据展示

  • WB analysis of PC-3 using 66941-1-Ig (same clone as 66941-1-PBS)

    WB analysis of PC-3 using 66941-1-Ig (same clone as 66941-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 66941-1-Ig (RDM1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66941-1-PBS in a different storage buffer formulation.

  • IHC staining of human thyroid cancer using 66941-1-Ig (same clone as 66941-1-PBS)

    IHC staining of human thyroid cancer using 66941-1-Ig (same clone as 66941-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66941-1-Ig (RDM1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66941-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 66941-1-Ig (same clone as 66941-1-PBS)

    IF Staining of A549 using 66941-1-Ig (same clone as 66941-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using RDM1 antibody (66941-1-Ig, Clone: 1C10B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66941-1-PBS in a different storage buffer formulation.

产品信息

66941-1-PBS targets RDM1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen RDM1 fusion protein Ag28686 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name RAD52 motif 1
Synonyms RAD52 homolog B, RAD52 motif 1, RAD52B, RDM1
Calculated Molecular Weight 284 aa, 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC038301
Gene Symbol RDM1
Gene ID (NCBI) 201299
RRIDAB_2882265
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8NG50
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RDM1, also named as RAD52B, encodes a RNA recognition motif (RRM)-containing protein confered resistance to the antitumor agent cisplatin. The expression of RDM1 is closely related to the cell proliferation and apoptosis of thyroid carcinoma cells and RDM1 may be a potential marker of lung adenocarcinoma. RDM1 has many isoforms (8 kDa, 12-19 kDa and 25-32 kDa) with long or short N-terminus and a great diversity in the exonic composition of C-terminus generated by alternative pre-mRNA splicing and differential usage of two translational start sites. RDM1 protein can be expressed differently in cytoplasm or nuclear/nucleolar in the absence of stress, proteotoxic stress and mild heat shock, suggesting functional differences among the RDM1 proteins. ( PMID: 28939762, 17905820, 28939762, 30069034)