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验证数据展示

  • WB analysis of PC-3 using 66941-1-Ig (same clone as 66941-1-PBS)

    WB analysis of PC-3 using 66941-1-Ig (same clone as 66941-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 66941-1-Ig (RDM1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66941-1-PBS in a different storage buffer formulation.

  • IHC staining of human thyroid cancer using 66941-1-Ig (same clone as 66941-1-PBS)

    IHC staining of human thyroid cancer using 66941-1-Ig (same clone as 66941-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66941-1-Ig (RDM1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66941-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 66941-1-Ig (same clone as 66941-1-PBS)

    IF Staining of A549 using 66941-1-Ig (same clone as 66941-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using RDM1 antibody (66941-1-Ig, Clone: 1C10B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66941-1-PBS in a different storage buffer formulation.

RDM1 Monoclonal antibody, PBS Only

RDM1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66941-1-PBS

产品说明书

CloneNo. 1C10B4

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse, Rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

RAD52 homolog B, RAD52 motif 1, RAD52B, RDM1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66941-1-PBS targets RDM1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 RDM1 fusion protein Ag28686 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 RAD52 motif 1
别名 RAD52 homolog B, RAD52 motif 1, RAD52B, RDM1
计算分子量 284 aa, 32 kDa
观测分子量 32 kDa
GenBank蛋白编号BC038301
基因名称 RDM1
Gene ID (NCBI) 201299
RRIDAB_2882265
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8NG50
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RDM1, also named as RAD52B, encodes a RNA recognition motif (RRM)-containing protein confered resistance to the antitumor agent cisplatin. The expression of RDM1 is closely related to the cell proliferation and apoptosis of thyroid carcinoma cells and RDM1 may be a potential marker of lung adenocarcinoma. RDM1 has many isoforms (8 kDa, 12-19 kDa and 25-32 kDa) with long or short N-terminus and a great diversity in the exonic composition of C-terminus generated by alternative pre-mRNA splicing and differential usage of two translational start sites. RDM1 protein can be expressed differently in cytoplasm or nuclear/nucleolar in the absence of stress, proteotoxic stress and mild heat shock, suggesting functional differences among the RDM1 proteins. ( PMID: 28939762, 17905820, 28939762, 30069034)