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验证数据展示

  • WB analysis using 13790-1-AP

    WB analysis using 13790-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13790-1-AP (Radixin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 13790-1-AP

    IHC staining of human pancreas cancer using 13790-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13790-1-AP (Radixin antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with Radixin/RDX antibody included
  • IHC staining of human pancreas cancer using 13790-1-AP

    IHC staining of human pancreas cancer using 13790-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13790-1-AP (Radixin antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with Radixin/RDX antibody included
  • IF Staining of MCF-7 using 13790-1-AP

    IF Staining of MCF-7 using 13790-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Radixin antibody (13790-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Radixin Polyclonal antibody

Radixin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13790-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Synonyms

RDX

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse pancreas tissue, Y79 cells, rat pancreas tissue
Positive IHC detected inhuman pancreas cancer tissue, human liver cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy Radixin/RDX Ready-To-Use IHC Kit. Radixin/RDX primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13790-1-AP targets Radixin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen Radixin fusion protein Ag4813 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name radixin
Synonyms RDX
Calculated Molecular Weight 583 aa, 68 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC047109
Gene Symbol RDX
Gene ID (NCBI) 5962
RRIDAB_2300889
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35241
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Radixin belongs to the ezrin-radixin-moesin (ERM) family of proteins which act as cross-linkers between membrane and actin cytoskeleton. ERM proteins provide structural links to strengthen the cell cortex and facilitate several key cellular processes, including membrane dynamics, substrate adhesion, cell survival, cell adhesion, and motility. The function of ERM proteins is highly reliant on phosphorylation induced conformational changes in response to chemokine and antigen stimulation.

实验方案

Product Specific Protocols
WB protocol for Radixin antibody 13790-1-APDownload protocol
IHC protocol for Radixin antibody 13790-1-APDownload protocol
IF protocol for Radixin antibody 13790-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Am J Pathol

Elevated Expression of Moesin in Muscular Dystrophies.

Authors - Mark Pines
View Article
humanWB

Drug Resist Updat

Intercellular adhesion molecule-1 suppresses TMZ chemosensitivity in acquired TMZ-resistant gliomas by increasing assembly of ABCB1 on the membrane

Authors - Xin Zhang
View Article