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  • Featured Product
  • KD/KO Validated

RECQL4 Polyclonal antibody

RECQL4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 17008-1-AP

Print datasheet

Synonyms

RecQ protein like 4, EC:5.6.2.4, DNA helicase, RecQ-like type 4, DNA 3'-5' helicase RecQ4, ATP-dependent DNA helicase Q4

验证数据展示

  • IHC staining of human gliomas using 17008-1-AP

    IHC staining of human gliomas using 17008-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 17008-1-AP (RECQL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RECQL4 antibody included
  • WB analysis using 17008-1-AP

    WB analysis using 17008-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17008-1-AP (RECQL4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 17008-1-AP

    IP experiment of HeLa using 17008-1-AP

    IP result of anti-RECQL4 (IP:17008-1-AP, 4ug; Detection:17008-1-AP 1:1000) with HeLa cells lysate 1880 ug.

  • IF Staining of HeLa using 17008-1-AP

    IF Staining of HeLa using 17008-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RECQL4 antibody (17008-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman gliomas tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RECQL4 Ready-To-Use IHC Kit. RECQL4 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17008-1-AP targets RECQL4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IP, IF
Tested Reactivity human
Cited Reactivityhuman
Immunogen RECQL4 fusion protein Ag10707 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RecQ protein-like 4
Synonyms RecQ protein like 4, EC:5.6.2.4, DNA helicase, RecQ-like type 4, DNA 3'-5' helicase RecQ4, ATP-dependent DNA helicase Q4
Calculated Molecular Weight 1208 aa, 133 kDa
Observed Molecular Weight 145-150 kDa
GenBank Accession NumberBC013277
Gene Symbol RECQL4
Gene ID (NCBI) 9401
RRIDAB_2238324
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94761
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RECQ protein-like 4(RECOL4), which has helicase domains contain 7 consensus motifs in the middle, is a gene responsible for Werner syndorme and Bloom syndrome. It forms a stable complex with UBR1 adn UBR2, which has a DNA-stimulated ATPase activity. RECOL4 may involve in repairment of DNA damage by interaction with APE1 and FEN1, key participants in base excision repair. This is a rabbit polyclonal antibody raised against part of C-terminal of RECQ4 of human origin. The calculated molecular weight of EIF3B is 133 kDa, but the modified EIF3B protein is about 145-150 kDa.

实验方案

Product Specific Protocols
WB protocol for RECQL4 antibody 17008-1-APDownload protocol
IHC protocol for RECQL4 antibody 17008-1-APDownload protocol
IF protocol for RECQL4 antibody 17008-1-APDownload protocol
IP protocol for RECQL4 antibody 17008-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Nat Commun

DNA replication initiation factor RECQ4 possesses a role in antagonizing DNA replication initiation

Authors - Xiaohua Xu
View Article
humanIHC

Cancer Biol Med

RECQL4 regulates DNA damage response and redox homeostasis in esophageal cancer.

Authors - Guosheng Lyu
  • KD Validated
View Article
humanWB

FASEB J

UBE2O and USP7 co-regulate RECQL4 ubiquitinylation and homologous recombination-mediated DNA repair.

Authors - Qiuling Huang
View Article
humanWB

J Biol Chem

The Deubiquitinase USP28 Stabilizes Expression of RecQ Family Helicases and Maintains the Viability of Triple Negative Breast Cancer Cells.

Authors - Jiewei Wang
View Article
humanIF

Sci Rep

PARP1 regulates DNA damage-induced nucleolar-nucleoplasmic shuttling of WRN and XRCC1 in a toxicant and protein-specific manner.

Authors - Sebastian Veith
View Article
humanIF

bioRxiv

USP50 suppresses alternative RecQ helicase use and deleterious DNA2 activity during replication

Authors - Hannah L Mackay
View Article