REDD1 Recombinant antibody

REDD1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 82650-1-RR

产品说明书

CloneNo. 1L2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

DDIT4, 1L2, RTP801, REDD-1, HIF-1 responsive protein RTP801

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA549 cells, Cobalt Chloride treated HeLa cells, K-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:14000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82650-1-RR targets REDD1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 REDD1 fusion protein Ag0965 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 DNA-damage-inducible transcript 4
别名 DDIT4, 1L2, RTP801, REDD-1, HIF-1 responsive protein RTP801
计算分子量 25 kDa
观测分子量 32-35 kDa
GenBank蛋白编号BC007714
基因名称 REDD1/DDIT4
Gene ID (NCBI) 54541
RRIDAB_3086496
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NX09
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

REDD1, also named as RTP801 and DDIT4, belongs to the DDIT4 family. REDD1 promotes neuronal cell death. It is a novel transcriptional target of p53 implicated ROS in the p53-dependent DNA damage response. REDD1 controlled cell growth under energy stress, as an essential regulator of TOR activity through the TSC1/2 complex. REDD-1 expression has also been linked to apoptosis, Aβ toxicity and the pathogenesis of ischemic diseases. As an HIF-1-responsive gene, REDD-1 exhibits strong hypoxia-dependent upregulation in ischemic cells of neuronal origin[PMID: 19996311]. In response to stress due to DNA damage and glucocorticoid treatment, REDD-1 is upregulated at the transcriptional level[PMID: 21733849]. REDD-1 negatively regulates the mammalian target of Rapamycin, a serine/threonine kinase often referred to as mTOR[PMID: 22951983]. It is crucial in the coupling of extra- and intracellular cues to mTOR regulation. The absence of REDD-1 is associated with the development of retinopathy, a major cause of blindness[PMID: 22304497]. REDD1 is a new host defense factor, and chemical activation of REDD1 expression represents a potent antiviral intervention strategy[PMID: 21909097]. The calculated molecular weight of REDD1 is 25 kDa. Because of multiple lysines in the proteins, REDD1 offen migrates around 35 kDa on Western blot[PMID: 19221489].

实验方案

Product Specific Protocols
WB protocol for REDD1 antibody 82650-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
Loading...