验证数据展示
经过测试的应用
| Positive WB detected in | BxPC-3 cells, HepG2 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | BxPC-3 cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, mouse olfactory epithelium tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12088-1-AP targets REEP6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | REEP6 fusion protein Ag2723 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | receptor accessory protein 6 |
| 别名 | Receptor expression-enhancing protein 6, Polyposis locus protein 1-like 1, DP1L1, C19orf32 |
| 计算分子量 | 201 aa, 22 kDa |
| 观测分子量 | 17-22 kDa |
| GenBank蛋白编号 | BC008201 |
| 基因名称 | REEP6 |
| Gene ID (NCBI) | 92840 |
| RRID | AB_2178460 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96HR9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for REEP6 antibody 12088-1-AP | Download protocol |
| IHC protocol for REEP6 antibody 12088-1-AP | Download protocol |
| IF protocol for REEP6 antibody 12088-1-AP | Download protocol |
| IP protocol for REEP6 antibody 12088-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Psychiatry Dysfunctional tubular endoplasmic reticulum constitutes a pathological feature of Alzheimer's disease. | ||
Hum Mol Genet REEP6 deficiency leads to retinal degeneration through disruption of ER homeostasis and protein trafficking. | ||
Int J Biol Macromol Receptor accessory protein 6, a novel ferroptosis suppressor, drives oral squamous cell carcinoma by maintaining endoplasmic reticulum hemostasis |
