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RELB Polyclonal antibody

RELB Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 29601-1-AP

Print datasheet

Synonyms

Transcription factor RelB, I-Rel

验证数据展示

  • WB analysis using 29601-1-AP

    WB analysis using 29601-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29601-1-AP (RELB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of Raji using 29601-1-AP

    IP experiment of Raji using 29601-1-AP

    IP result of anti-RELB (IP:29601-1-AP, 4ug; Detection:29601-1-AP 1:1000) with Raji cells lysate 2840 ug.

  • IHC staining of human ovary cancer using 29601-1-AP

    IHC staining of human ovary cancer using 29601-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 29601-1-AP (RELB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RELB antibody included

经过测试的应用

Positive WB detected inRaji cells, Ramos cells
Positive IP detected inRaji cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RELB Ready-To-Use IHC Kit. RELB primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29601-1-AP targets RELB in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Tested Reactivity human
Immunogen RELB fusion protein Ag30334 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name v-rel reticuloendotheliosis viral oncogene homolog B
Synonyms Transcription factor RelB, I-Rel
Calculated Molecular Weight 62 kDa
Observed Molecular Weight 62-65 kDa
GenBank Accession NumberBC028013
Gene Symbol RELB
Gene ID (NCBI) 5971
RRIDAB_2918330
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ01201
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RELB is one of the more unusual members of the NF-κB family, it is best known for its roles in lymphoid development, DC biology, and noncanonical signaling. RELB is capable of direct binding to the AhR that supports the xenobiotic-detoxifying pathway. RELB can regulate the circadian rhythm by directly binding to the BMAL partner of CLOCK.

实验方案

Product Specific Protocols
WB protocol for RELB antibody 29601-1-APDownload protocol
IHC protocol for RELB antibody 29601-1-APDownload protocol
IP protocol for RELB antibody 29601-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols