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RELL1 Polyclonal antibody

RELL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29823-1-AP

Print datasheet

Synonyms

FLJ21778, RELL1, RELT like 1, RELT like protein 1

验证数据展示

  • WB analysis of MCF-7 using 29823-1-AP

    WB analysis of MCF-7 using 29823-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 29823-1-AP (RELL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat skin using 29823-1-AP

    IHC staining of rat skin using 29823-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 29823-1-AP (RELL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inMCF-7 cells
Positive IHC detected inrat skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29823-1-AP targets RELL1 in WB, IHC, ELISA applications and shows reactivity with Human, Rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, Rat
Immunogen RELL1 fusion protein Ag31475 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RELT-like 1
Synonyms FLJ21778, RELL1, RELT like 1, RELT like protein 1
Observed Molecular Weight29 kDa
GenBank Accession NumberBC039540
Gene Symbol RELL1
Gene ID (NCBI) 768211
RRIDAB_3086175
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IUW5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RELT-like protein 1 (RELL1) is a member of the tumor necrosis factor (TNF) receptor family and belongs to the receptor expressed in the lymphoid tissues (RELT) family. RELL1 activates the pro-inflammatory pathway by binding to TNF receptor-related factor 1 (TRAF1). Further, RELT family members activate p38 and induce apoptosis by a mechanism distinct from that of TNF receptors (PMID: 32090861). The molecular weight of RELL1 is 29 kDa.

实验方案

Product Specific Protocols
WB protocol for RELL1 antibody 29823-1-APDownload protocol
IHC protocol for RELL1 antibody 29823-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols