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验证数据展示

  • WB analysis of MCF-7 using 29823-1-AP

    WB analysis of MCF-7 using 29823-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 29823-1-AP (RELL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat skin using 29823-1-AP

    IHC staining of rat skin using 29823-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 29823-1-AP (RELL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RELL1 Polyclonal antibody

RELL1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29823-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

Human, Rat and More (1)

应用

WB, IHC, ELISA

别名

FLJ21778, RELL1, RELT like 1, RELT like protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells
Positive IHC detected inrat skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

29823-1-AP targets RELL1 in WB, IHC, ELISA applications and shows reactivity with Human, Rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 Human, Rat
文献引用反应性human
免疫原 RELL1 fusion protein Ag31475 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RELT-like 1
别名 FLJ21778, RELL1, RELT like 1, RELT like protein 1
观测分子量29 kDa
GenBank蛋白编号BC039540
基因名称 RELL1
Gene ID (NCBI) 768211
RRIDAB_3086175
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IUW5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RELT-like protein 1 (RELL1) is a member of the tumor necrosis factor (TNF) receptor family and belongs to the receptor expressed in the lymphoid tissues (RELT) family. RELL1 activates the pro-inflammatory pathway by binding to TNF receptor-related factor 1 (TRAF1). Further, RELT family members activate p38 and induce apoptosis by a mechanism distinct from that of TNF receptors (PMID: 32090861). The molecular weight of RELL1 is 29 kDa.

实验方案

Product Specific Protocols
WB protocol for RELL1 antibody 29823-1-APDownload protocol
IHC protocol for RELL1 antibody 29823-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

bioRxiv

Leucine Aminopeptidase LyLAP enables lysosomal degradation of membrane proteins

Authors - Aakriti Jain
View Article