• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Renin Polyclonal antibody

Renin Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, monkey

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 29330-1-AP

Print datasheet

Synonyms

Angiotensinogenase, FLJ10761, REN, Renin

验证数据展示

  • WB analysis using 29330-1-AP

    WB analysis using 29330-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29330-1-AP (Renin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inCOS-7 cells, HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29330-1-AP targets Renin in WB, ELISA applications and shows reactivity with Human, monkey samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, monkey
Immunogen Renin fusion protein Ag31071 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name renin
Synonyms Angiotensinogenase, FLJ10761, REN, Renin
Calculated Molecular Weight 45 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC047752
Gene Symbol REN
Gene ID (NCBI) 5972
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP00797
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Renin is an aspartic protease that consists of 2 homologous lobes, produced as pre-prorenin protein and transferred into the cisterns of the endoplasmic reticulum. Renin is mainly produced and released into circulation by the so-called juxtaglomerular epithelioid cells, located in the walls of renal afferent arterioles at the entrance of the glomerular capillary network (PMID: 16525949).REN has 2 isoforms produced by alternative splicing with a molecular weight of 45-47kDa.

实验方案

Product Specific Protocols
WB protocol for Renin antibody 29330-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols