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验证数据展示

  • WB analysis using 29330-1-AP

    WB analysis using 29330-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29330-1-AP (Renin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 29330-1-AP

    IF Staining of HepG2 using 29330-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Renin antibody (29330-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Renin Polyclonal antibody

Renin Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 29330-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey

Applications

WB, IF/ICC, ELISA

Synonyms

REN, EC:3.4.23.15, Angiotensinogenase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inCOS-7 cells, HepG2 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29330-1-AP targets Renin in WB, IF/ICC, ELISA applications and shows reactivity with human, monkey samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, monkey
Immunogen Renin fusion protein Ag31071 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name renin
Synonyms REN, EC:3.4.23.15, Angiotensinogenase
Calculated Molecular Weight 45 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC047752
Gene Symbol Renin
Gene ID (NCBI) 5972
RRIDAB_3086119
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP00797
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Renin is an aspartic protease that consists of 2 homologous lobes, produced as pre-prorenin protein and transferred into the cisterns of the endoplasmic reticulum. Renin is mainly produced and released into circulation by the so-called juxtaglomerular epithelioid cells, located in the walls of renal afferent arterioles at the entrance of the glomerular capillary network (PMID: 16525949).REN has 2 isoforms produced by alternative splicing with a molecular weight of 45-47kDa.

实验方案

Product Specific Protocols
WB protocol for Renin antibody 29330-1-APDownload protocol
IF protocol for Renin antibody 29330-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols