• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of Jurkat using 19159-1-AP

    WB analysis of Jurkat using 19159-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19159-1-AP (RFC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 19159-1-AP

    WB analysis of HeLa using 19159-1-AP

    WB result of RFC1 antibody (19159-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RFC1 transfected HeLa cells.

  • Featured Product
  • KD/KO Validated

RFC1 Polyclonal antibody

RFC1 Polyclonal Antibody for WB, ELISA
Cat No. 19159-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

DNA binding protein PO GA, Activator 1 subunit 1, Activator 1 large subunit, Activator 1 140 kDa subunit, A1 140 kDa subunit

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inJurkat cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19159-1-AP targets RFC1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen RFC1 fusion protein Ag13585 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name replication factor C (activator 1) 1, 145kDa
Synonyms DNA binding protein PO GA, Activator 1 subunit 1, Activator 1 large subunit, Activator 1 140 kDa subunit, A1 140 kDa subunit
Calculated Molecular Weight 128 kDa
Observed Molecular Weight 128-150 kDa
GenBank Accession NumberBC051751
Gene Symbol RFC1
Gene ID (NCBI) 5981
RRIDAB_2878574
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP35251
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Replication factor C subunit 1 (RFC1) is a pentameric DNA polymerase accessory protein involved in DNA replication and repair (PMID: 10051561). It was reported as a cause of cerebellar ataxia with neuropathy and vestibular areflexia syndrome (CANVAS) (PMID: 30926972).

实验方案

Product Specific Protocols
WB protocol for RFC1 antibody 19159-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols