验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells, PC-3 cells, Raji cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
产品信息
10410-1-AP targets RFC2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | RFC2 fusion protein Ag0663 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | replication factor C (activator 1) 2, 40kDa |
| 别名 | RF C 40 kDa subunit, Replication factor C subunit 2, Activator 1 subunit 2, Activator 1 40 kDa subunit, A1 40 kDa subunit |
| 计算分子量 | 39 kDa |
| 观测分子量 | 38 kDa |
| GenBank蛋白编号 | BC002813 |
| 基因名称 | RFC2 |
| Gene ID (NCBI) | 5982 |
| RRID | AB_2285035 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P35250 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RFC2, also named as RFC40, belongs to the activator 1 small subunits family. It is a 40kd Activator 1 subunit. It binds ATP. The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. RFC2 is a constitutively expressed protein essential for both DNA repair and replication. (PMID: 20660762).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RFC2 antibody 10410-1-AP | Download protocol |
| IF protocol for RFC2 antibody 10410-1-AP | Download protocol |
| IP protocol for RFC2 antibody 10410-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Syst Biol The MHC I immunopeptidome conveys to the cell surface an integrative view of cellular regulation. | ||
Proc Natl Acad Sci U S A Deletion of TDP-43 down-regulates Tbc1d1, a gene linked to obesity, and alters body fat metabolism. | ||
Sci Rep RFC2: a prognosis biomarker correlated with the immune signature in diffuse lower-grade gliomas. | ||
bioRxiv Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA polyubiquitination | ||
Cell Rep Arginine shortage induces replication stress and confers genotoxic resistance by inhibiting histone H4 translation and promoting PCNA ubiquitination |