- Featured Product
- KD/KO Validated
RFC3 Polyclonal antibody
RFC3 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | human liver tissue, HeLa cells, human heart tissue |
Positive IP detected in | HeLa cells |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 1 publications below |
WB | See 4 publications below |
CoIP | See 1 publications below |
产品信息
11814-1-AP targets RFC3 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, CoIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | RFC3 fusion protein Ag2269 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | replication factor C (activator 1) 3, 38kDa |
Synonyms | RF-C 38 kDa subunit |
Calculated Molecular Weight | 40 aa, 6 kDa |
Observed Molecular Weight | 38 kDa |
GenBank Accession Number | BC000149 |
Gene Symbol | RFC3 |
Gene ID (NCBI) | 5983 |
RRID | AB_2178470 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P40938 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for RFC3 antibody 11814-1-AP | Download protocol |
IF protocol for RFC3 antibody 11814-1-AP | Download protocol |
IP protocol for RFC3 antibody 11814-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Acta Biochim Biophys Sin (Shanghai) FANCA facilitates G1/S cell cycle advancement, proliferation, migration and invasion in gastric cancer | ||
Virol J IRF2 inhibits ZIKV replication by promoting FAM111A expression to enhance the host restriction effect of RFC3.
| ||
Cell Rep Cyclin N-Terminal Domain-Containing-1 Coordinates Meiotic Crossover Formation with Cell-Cycle Progression in a Cyclin-Independent Manner. | ||
Exp Ther Med RFC3 drives the proliferation, migration, invasion and angiogenesis of colorectal cancer cells by binding KIF14 |