• Featured Product
  • KD/KO Validated

RFC3 Polyclonal antibody

RFC3 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 11814-1-AP

Print datasheet

Synonyms

RF-C 38 kDa subunit



经过测试的应用

Positive WB detected inhuman liver tissue, HeLa cells, human heart tissue
Positive IP detected inHeLa cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11814-1-AP targets RFC3 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen RFC3 fusion protein Ag2269 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name replication factor C (activator 1) 3, 38kDa
Synonyms RF-C 38 kDa subunit
Calculated Molecular Weight 40 aa, 6 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC000149
Gene Symbol RFC3
Gene ID (NCBI) 5983
RRIDAB_2178470
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP40938
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RFC3, also known as RFC38, is a 356 amino acid protein, which localizes in nucleus. Replication factor C (RFC) is a component of the eukaryotic DNA polymerase . In all eukaryotic cell cycles, it is essential for DNA duplication, DNA injury repair and checkpoint control. RFC consists of five subunits (RFC1-5). The interrelationships between RFC2, RFC3 and the c-MYC oncogene (transcription factor) can induce cell division and proliferation. RFC3 is associated with liver, breast, esophageal and ovarian cancer, and serves a critical role in cellular proliferation, invasion and metastasis. RFC3 exists two isoforms with molecular weight of 34 kDa and 38-40 kDa.

实验方案

Product Specific Protocols
WB protocol for RFC3 antibody 11814-1-APDownload protocol
IF protocol for RFC3 antibody 11814-1-APDownload protocol
IP protocol for RFC3 antibody 11814-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Acta Biochim Biophys Sin (Shanghai)

FANCA facilitates G1/S cell cycle advancement, proliferation, migration and invasion in gastric cancer

Authors - Wei Wang
humanWB

Virol J

IRF2 inhibits ZIKV replication by promoting FAM111A expression to enhance the host restriction effect of RFC3.

Authors - Kai Ren
  • KD Validated
mouseWB

Cell Rep

Cyclin N-Terminal Domain-Containing-1 Coordinates Meiotic Crossover Formation with Cell-Cycle Progression in a Cyclin-Independent Manner.

Authors - Stephen Gray
humanWB

Cell Rep

TRPS1 drives heterochromatic origin refiring and cancer genome evolution.

Authors - Jianguo Yang
humanCoIP

Exp Ther Med

RFC3 drives the proliferation, migration, invasion and angiogenesis of colorectal cancer cells by binding KIF14

Authors - Rong Yu