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验证数据展示

  • IF Staining of HeLa using 28671-1-AP

    IF Staining of HeLa using 28671-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RFC5 antibody (28671-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 28671-1-AP

    WB analysis using 28671-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28671-1-AP (RFC5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

RFC5 Polyclonal antibody

RFC5 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 28671-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

A1 36 kDa subunit, Activator 1 36 kDa subunit, Activator 1 subunit 5, Replication factor C subunit 5, RF C 36 kDa subunit, RFC36, RFC5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHCT 116 cells, U-251 cells, HEK-293T cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28671-1-AP targets RFC5 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 RFC5 fusion protein Ag29236 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 replication factor C (activator 1) 5, 36.5kDa
别名 A1 36 kDa subunit, Activator 1 36 kDa subunit, Activator 1 subunit 5, Replication factor C subunit 5, RF C 36 kDa subunit, RFC36, RFC5
计算分子量 36 aa, 5 kDa
GenBank蛋白编号BC001866
基因名称 RFC5
Gene ID (NCBI) 5985
RRIDAB_2918189
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP40937
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Replication factor C subunit 5 (RFC5), also known as activator 1, can recruit proliferating cell nuclear antigen(PCNA) onto the DNA polymerase S-phase checkpoint complex. In eukaryotes, RFC5 is involved in repairing mismatches, DNA double helix damage, nucleotide excision, and regulating the cell cycle(PMID: 30210909). RFC5 is significantly upregulated in cancer tissues or cells, and its expression is elevated with the cancer progression(PMID: 30214556).

实验方案

Product Specific Protocols
WB protocol for RFC5 antibody 28671-1-APDownload protocol
IF protocol for RFC5 antibody 28671-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols