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验证数据展示

  • WB analysis of MCF-7 using 85032-2-RR

    WB analysis of MCF-7 using 85032-2-RR

    WB result of RFK antibody (85032-2-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RFK transfected MCF-7 cells.

  • WB analysis using 85032-2-RR

    WB analysis using 85032-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85032-2-RR (RFK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

RFK Recombinant monoclonal antibody

RFK Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 85032-2-RR

产品说明书

CloneNo. 242628A4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

ATP:riboflavin 5'-phosphotransferase, EC:2.7.1.26, Flavokinase, Riboflavin kinase, RIFK

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, HEK-293 cells, U-87 MG cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85032-2-RR targets RFK in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag8591

Product name: Recombinant human RFK protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-162 aa of BC007069

Sequence: MPRADCIMRHLPYFCRGQVVRGFGRGSKQLGIPTANFPEQVVDNLPADISTGIYYGWASVGSGDVHKMVVSIGWNPYYKNTKKSMETHIMHTFKEDFYGEILNVAIVGYLRPEKNFDSLESLISAIQGDIEEAKKRLELPEHLKIKEDNFFQVSKSKIMNGH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 riboflavin kinase
别名 ATP:riboflavin 5'-phosphotransferase, EC:2.7.1.26, Flavokinase, Riboflavin kinase, RIFK
计算分子量 162 aa, 18 kDa
GenBank蛋白编号BC007069
基因名称 RFK
Gene ID (NCBI) 55312
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ969G6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Riboflavin kinase (RFK) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis(PMID:12623014). RFK is rate-limiting in the synthesis of FAD, an essential prosthetic group of NADPH oxidase.

实验方案

Product Specific Protocols
WB protocol for RFK antibody 85032-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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