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  • Featured Product
  • KD/KO Validated

RFX1 Polyclonal antibody

RFX1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 26859-1-AP

Print datasheet

Synonyms

EF C, Enhancer factor C, Regulatory factor X 1, RFX, RFX1, Transcription factor RFX1

验证数据展示

  • WB analysis of Jurkat using 26859-1-AP

    WB analysis of Jurkat using 26859-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 26859-1-AP (RFX1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 26859-1-AP

    IP experiment of Jurkat using 26859-1-AP

    IP result of anti-RFX1 (IP:26859-1-AP, 4ug; Detection:26859-1-AP 1:500) with Jurkat cells lysate 2030 ug.

经过测试的应用

Positive WB detected inJurkat cells, HL-60 cells
Positive IP detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26859-1-AP targets RFX1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen RFX1 fusion protein Ag25442 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name regulatory factor X, 1 (influences HLA class II expression)
Synonyms EF C, Enhancer factor C, Regulatory factor X 1, RFX, RFX1, Transcription factor RFX1
Calculated Molecular Weight 105 kDa
Observed Molecular Weight 135 kDa
GenBank Accession NumberBC049826
Gene Symbol RFX1
Gene ID (NCBI) 5989
RRIDAB_2880659
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP22670
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RFX1, also named as MHC class II regulatory factor RFX1, is a 979 amino acid protein which contains 1 RFX-type winged-helix DNA-binding domain and belongs to the RFX family. RFX1 can binds DNA as a homodimer and heterodimerizes with RFX2 and RFX3. RFX1 as a regulatory factor is essential for MHC class II genes expression. RFX1 protein is modified after translation and its molecular weight could be migrate to 130-140 kDa (PMID: 20189986 ).

实验方案

Product Specific Protocols
WB protocol for RFX1 antibody 26859-1-APDownload protocol
IP protocol for RFX1 antibody 26859-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanIF,WB

Int J Biol Sci

Hypoxia-induced exosomes facilitate lung pre-metastatic niche formation in hepatocellular carcinoma through the miR-4508-RFX1-IL17A-p38 MAPK-NF-κB pathway

Authors - Wentao Jia
  • KD Validated
View Article
human,mouseIHC

Ren Fail

Proteomics reveals the key transcription-related factors mediating obstructive nephropathy in pediatric patients and mice

Authors - Hualin Cao
View Article