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RFX3 Polyclonal antibody

RFX3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 14784-1-AP

Print datasheet

Synonyms

Regulatory factor X 3, RFX3, Transcription factor RFX3

验证数据展示

  • WB analysis of HeLa using 14784-1-AP

    WB analysis of HeLa using 14784-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14784-1-AP (RFX3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 14784-1-AP

    IP experiment of HeLa using 14784-1-AP

    IP result of anti-RFX3 (IP:14784-1-AP, 4ug; Detection:14784-1-AP 1:500) with HeLa cells lysate 1400ug.

经过测试的应用

Positive WB detected inHeLa cells, K-562 cells
Positive IP detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14784-1-AP targets RFX3 in WB, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB, ChIP
Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Immunogen RFX3 fusion protein Ag6413 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name regulatory factor X, 3 (influences HLA class II expression)
Synonyms Regulatory factor X 3, RFX3, Transcription factor RFX3
Calculated Molecular Weight 84 kDa
Observed Molecular Weight 70-79 kDa
GenBank Accession NumberBC067778
Gene Symbol RFX3
Gene ID (NCBI) 5991
RRIDAB_2178917
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48380
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RFX3 antibody 14784-1-APDownload protocol
IP protocol for RFX3 antibody 14784-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratChIP

Nat Commun

Distinct regulation of hippocampal neuroplasticity and ciliary genes by corticosteroid receptors.

Authors - Karen R Mifsud
View Article
mouseWB

Aging (Albany NY)

RNA-seq analysis of the key long noncoding RNAs and mRNAs related to cognitive impairment after cardiac arrest and cardiopulmonary resuscitation.

Authors - Chan Chen
View Article