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RFX4 Polyclonal antibody

RFX4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13971-1-AP

Print datasheet

Synonyms

NYD SP10, Regulatory factor X 4, RFX4, Transcription factor RFX4

验证数据展示

  • WB analysis using 13971-1-AP

    WB analysis using 13971-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13971-1-AP (RFX4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13971-1-AP targets RFX4 in WB, ELISA applications and shows reactivity with mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity mouse, rat
Immunogen RFX4 fusion protein Ag5004 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name regulatory factor X, 4 (influences HLA class II expression)
Synonyms NYD SP10, Regulatory factor X 4, RFX4, Transcription factor RFX4
Calculated Molecular Weight 83 kDa
Observed Molecular Weight84 kDa
GenBank Accession NumberBC030644
Gene Symbol RFX4
Gene ID (NCBI) 5992
RRIDAB_3085427
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ33E94
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RFX4, also named as NYD-SP10, contains one RFX-type winged-helix DNA-binding domain and belongs to the RFX family. It may activate transcription by interacting directly with the X-box The protein is structurally related to regulatory factors X1, X2, X3, and X5. It has been shown to interact with itself as well as with regulatory factors X2 and X3, but it does not interact with regulatory factor X1. This is a rabbit polyclonal antibody raised against the C terminus of human RFX4.

实验方案

Product Specific Protocols
WB protocol for RFX4 antibody 13971-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols