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RFXAP Polyclonal antibody

RFXAP Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20188-1-AP

Print datasheet

Synonyms

RFX associated protein, RFXAP

验证数据展示

  • WB analysis of HeLa using 20188-1-AP

    WB analysis of HeLa using 20188-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20188-1-AP (RFXAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 20188-1-AP

    IF Staining of HeLa using 20188-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 20188-1-AP (RFXAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 20188-1-AP is RFXAP Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen RFXAP fusion protein Ag14100 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name regulatory factor X-associated protein
Synonyms RFX associated protein, RFXAP
Calculated Molecular Weight 272 aa, 28 kDa
Observed Molecular Weight 23-26 kDa
GenBank Accession NumberBC026088
Gene Symbol RFXAP
Gene ID (NCBI) 5994
RRIDAB_10697835
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00287
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RFXAP is a subunit of the RFX complex that binds to the X-box of MHC II promoters. It's involved in Bare lymphocyte syndrome 2 (BLS2).

实验方案

Product Specific Protocols
WB protocol for RFXAP antibody 20188-1-APDownload protocol
IF protocol for RFXAP antibody 20188-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols