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  • Featured Product
  • KD/KO Validated

RGC32 Polyclonal antibody

RGC32 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 19556-1-AP

Print datasheet

Synonyms

C13orf15, bA157L14.2, RGCC, RGC-32, RGC 32

验证数据展示

  • WB analysis using 19556-1-AP

    WB analysis using 19556-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19556-1-AP (RGC32 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 19556-1-AP

    IHC staining of mouse kidney using 19556-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19556-1-AP (RGC32 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RGC32 antibody included
  • IHC staining of mouse kidney using 19556-1-AP

    IHC staining of mouse kidney using 19556-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 19556-1-AP (RGC32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RGC32 antibody included
  • IF Staining of Caco-2 using 19556-1-AP

    IF Staining of Caco-2 using 19556-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using RGC32 antibody (19556-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inmouse brain tissue, mouse liver tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCaco-2 cells
Planning an IHC experiment? We recommend our IHCeasy RGC32 Ready-To-Use IHC Kit. RGC32 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19556-1-AP targets RGC32 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromosome 13 open reading frame 15
Synonyms C13orf15, bA157L14.2, RGCC, RGC-32, RGC 32
Calculated Molecular Weight 15 kDa
Observed Molecular Weight13-15 kDa
GenBank Accession NumberNM_014059
Gene Symbol RGC32
Gene ID (NCBI) 28984
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H4X1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RGC32, also named as C13orf15, modulates the activity of cell cycle-specific kinases. RGC32 enhances CDC2 activity. RGC32 may contribute to the regulation of the cell cycle. And it inhibits growth of glioma cells by promoting arrest of mitotic progression at the G2/M transition. The antibody can recognize these 2 isoforms of RGC32.

实验方案

Product Specific Protocols
WB protocol for RGC32 antibody 19556-1-APDownload protocol
IHC protocol for RGC32 antibody 19556-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

J Immunol

RGC32 Promotes Bleomycin-Induced Systemic Sclerosis in a Murine Disease Model by Modulating Classically Activated Macrophage Function.

Authors - Chenming Sun
  • KO Validated
View Article