验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, mouse small intestine tissue, rat liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | rat kidney tissue, human liver tissue, human kidney tissue, human hepatocirrhosis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:3000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17947-1-AP targets RGN/SMP30 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, bovine |
| 免疫原 | RGN/SMP30 fusion protein Ag12423 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | regucalcin (senescence marker protein-30) |
| 别名 | RC, Regucalcin, RGN, RGN/SMP30, Senescence marker protein 30, SMP 30, SMP30 |
| 计算分子量 | 299 aa, 33 kDa |
| 观测分子量 | 33 kDa, 25 kDa |
| GenBank蛋白编号 | BC073173 |
| 基因名称 | RGN/SMP30 |
| Gene ID (NCBI) | 9104 |
| RRID | AB_10644486 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15493 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Regucalcin (RGN), also known as SMP30, is a highly conserved, calcium-binding protein, that is preferentially expressed in the liver and kidney. SMP30 is preferentially localized in hepatocytes and renal proximal tubular epithelial cells. It is known to be related to aging, hepatocyte proliferation and tumorigenesis. It may be useful for HCC serologic screening, especially for the patients with AFP Negative. RGN encodes two isoforms: 33 kDa and 25 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RGN/SMP30 antibody 17947-1-AP | Download protocol |
| IHC protocol for RGN/SMP30 antibody 17947-1-AP | Download protocol |
| IP protocol for RGN/SMP30 antibody 17947-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Transl Lung Cancer Res Senescence marker protein 30 inhibits tumor growth by reducing HDAC4 expression in non-small cell lung cancer. | ||
Ecotoxicol Environ Saf Hexavalent chromium triggers hepatocytes premature senescence via the GATA4/NF-κB signaling pathway mediated by the DNA damage response. | ||
Ecotoxicol Environ Saf Identification and functional analysis of senescence-associated secretory phenotype of premature senescent hepatocytes induced by hexavalent chromium. | ||
J Endocrinol Hepatic thyroid signaling of heat-stressed late pregnant and early lactating cows. | ||
Int Immunopharmacol Senescence marker protein 30 (SMP30) protects against high glucose-induced apoptosis, oxidative stress and inflammatory response in retinal ganglion cells by enhancing Nrf2 activation via regulation of Akt/GSK-3β pathway | ||
Toxicol Lett Dioscin, a natural steroid saponin, shows remarkable protective effect against acetaminophen-induced liver damage in vitro and in vivo. |
