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ARHGEF28 Polyclonal antibody

ARHGEF28 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 17144-1-AP

Print datasheet

Synonyms

RGNEF, Rho guanine nucleotide exchange factor 28, p190-RhoGEF, p190RhoGEF, p190 RhoGEF

验证数据展示

  • WB analysis using 17144-1-AP

    WB analysis using 17144-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17144-1-AP (RGNEF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17144-1-AP targets ARHGEF28 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen ARHGEF28 fusion protein Ag10915 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Rho-guanine nucleotide exchange factor
Synonyms RGNEF, Rho guanine nucleotide exchange factor 28, p190-RhoGEF, p190RhoGEF, p190 RhoGEF
Calculated Molecular Weight 192 kDa
Observed Molecular Weight192 kDa
GenBank Accession NumberBC012946
Gene Symbol RGNEF
Gene ID (NCBI) 64283
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N1W1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARHGEF28, also known as RGNEF or p190RhoGEF, is a 190 kDa RNA binding protein (RBP) that contains a Dbl/PH domain capable of RhoA activation. ARHGEF28 interacts with low molecular weight neurofilament mRNA and may be involved in the formation of amyotrophic lateral sclerosis neurofilament aggregates.

实验方案

Product Specific Protocols
WB protocol for ARHGEF28 antibody 17144-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols