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验证数据展示

  • WB analysis using 17144-1-AP

    WB analysis using 17144-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17144-1-AP (RGNEF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

ARHGEF28 Polyclonal antibody

ARHGEF28 Polyclonal Antibody for WB, ELISA
Cat No. 17144-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

RGNEF, Rho guanine nucleotide exchange factor 28, p190-RhoGEF, p190RhoGEF, p190 RhoGEF

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17144-1-AP targets ARHGEF28 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 ARHGEF28 fusion protein Ag10915 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Rho-guanine nucleotide exchange factor
别名 RGNEF, Rho guanine nucleotide exchange factor 28, p190-RhoGEF, p190RhoGEF, p190 RhoGEF
计算分子量 192 kDa
观测分子量192 kDa
GenBank蛋白编号BC012946
基因名称 RGNEF
Gene ID (NCBI) 64283
RRIDAB_3669262
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N1W1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ARHGEF28, also known as RGNEF or p190RhoGEF, is a 190 kDa RNA binding protein (RBP) that contains a Dbl/PH domain capable of RhoA activation. ARHGEF28 interacts with low molecular weight neurofilament mRNA and may be involved in the formation of amyotrophic lateral sclerosis neurofilament aggregates.

实验方案

Product Specific Protocols
WB protocol for ARHGEF28 antibody 17144-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols