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  • Featured Product
  • KD/KO Validated

RGS10 Polyclonal antibody

RGS10 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11163-1-AP

Print datasheet

Synonyms

Regulator of G-protein signaling 10

验证数据展示

  • WB analysis of mouse thymus using 11163-1-AP

    WB analysis of mouse thymus using 11163-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11163-1-AP (RGS10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 11163-1-AP

    IHC staining of human stomach using 11163-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11163-1-AP (RGS10 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse thymus tissue, K-562 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11163-1-AP targets RGS10 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen RGS10 fusion protein Ag1644 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name regulator of G-protein signaling 10
Synonyms Regulator of G-protein signaling 10
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 20-22 kDa
GenBank Accession NumberBC009361
Gene Symbol RGS10
Gene ID (NCBI) 6001
RRIDAB_10596933
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43665
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RGS10 antibody 11163-1-APDownload protocol
IHC protocol for RGS10 antibody 11163-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Int Endod J

RGS10 negatively regulates apical periodontitis via TFEB-mediated autophagy in BABL/c mice model and in vitro

Authors - Jiaxin Li
View Article
mouseWB

Oral Dis

Inhibition of Rgs10 aggravates periodontitis with collagen-induced arthritis via the NF-κB pathway.

Authors - Wei-Cheng Chan
  • KD Validated
View Article
mouseWB

J Endod

Inhibition of RGS10 Aggravates Periapical Periodontitis via Upregulation of the NF-κB Pathway

Authors - Wei Wei
View Article