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验证数据展示

  • IHC staining of human testis using 12549-1-AP

    IHC staining of human testis using 12549-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 12549-1-AP (RGS17 Antibody) at dilution of 1:50.

  • IHC staining of human testis using 12549-1-AP

    IHC staining of human testis using 12549-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis slide using 12549-1-AP (RGS17 Antibody) at dilution of 1:50.

  • IF Staining of MCF-7 using 12549-1-AP

    IF Staining of MCF-7 using 12549-1-AP

    Immunofluorescent analysis of MCF-7 cells using 12549-1-AP (RGS17 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 12549-1-AP

    WB analysis using 12549-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12549-1-AP (RGS17 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

RGS17 Polyclonal antibody

RGS17 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12549-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

RGSZ2, RGS 17, Regulator of G-protein signaling 17, hRGS17

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, PC-3 cells, SH-SY5Y cells, mouse brain tissue, mouse lung tissue, MDA-MB-231 cells, NCI-H1299 cells, SK-BR-3 cells
Positive IHC detected inhuman testis tissue, human cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12549-1-AP targets RGS17 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human, rat
免疫原 RGS17 fusion protein Ag3399 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 regulator of G-protein signaling 17
别名 RGSZ2, RGS 17, Regulator of G-protein signaling 17, hRGS17
计算分子量 210 aa, 24 kDa
观测分子量 27 kDa
GenBank蛋白编号BC013117
基因名称 RGS17
Gene ID (NCBI) 26575
RRIDAB_2179954
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UGC6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RGS17 antibody 12549-1-APDownload protocol
IHC protocol for RGS17 antibody 12549-1-APDownload protocol
IF protocol for RGS17 antibody 12549-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Signal

Altered expression and function of regulator of G-protein signaling-17 (RGS17) in hepatocellular carcinoma.

Authors - Eugene Sokolov
View Article
ratWB,IF

Sci Rep

Regulator of G protein signaling 17 represents a novel target for treating cisplatin induced hearing loss.

Authors - Asmita Dhukhwa
View Article
humanWB, IHC

J Cancer

Deregulation of RGS17 Expression Promotes Breast Cancer Progression.

Authors - Yuhua Li
View Article