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验证数据展示

  • WB analysis using 27394-1-AP

    WB analysis using 27394-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27394-1-AP (RGS8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 27394-1-AP

    IHC staining of mouse cerebellum using 27394-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27394-1-AP (RGS8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 27394-1-AP

    IHC staining of mouse cerebellum using 27394-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27394-1-AP (RGS8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse cerebellum using 27394-1-AP

    IF Staining of mouse cerebellum using 27394-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using RGS8 antibody (27394-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse cerebellum using 27394-1-AP

    IF Staining of mouse cerebellum using 27394-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using RGS8 antibody (27394-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse cerebellum using 27394-1-AP

    IHC staining of mouse cerebellum using 27394-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27394-1-AP (RGS8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 27394-1-AP

    IHC staining of mouse cerebellum using 27394-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27394-1-AP (RGS8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RGS8 Polyclonal antibody

RGS8 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 27394-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

Regulator of G-protein signaling 8

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse liver tissue, rat brain tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27394-1-AP targets RGS8 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RGS8 fusion protein Ag26535 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name regulator of G-protein signaling 8
Synonyms Regulator of G-protein signaling 8
Calculated Molecular Weight 198 aa, 23 kDa
Observed Molecular Weight21 kDa
GenBank Accession NumberBC069718
Gene Symbol RGS8
Gene ID (NCBI) 85397
RRIDAB_3085953
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP57771
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RGS8 is a member of the regulator of G protein signaling (RGS) family and encodes a protein with a single RGS domain. Regulator of G protein signaling (RGS) proteins are regulatory and structural components of G protein-coupled receptor complexes. They accelerate transit through the cycle of GTP binding and hydrolysis to GDP, thereby terminating signal transduction, but paradoxically, also accelerate receptor-stimulated activation.

实验方案

Product Specific Protocols
WB protocol for RGS8 antibody 27394-1-APDownload protocol
IHC protocol for RGS8 antibody 27394-1-APDownload protocol
IF protocol for RGS8 antibody 27394-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols