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验证数据展示

  • WB analysis of human placenta using 67714-1-Ig

    WB analysis of human placenta using 67714-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67714-1-Ig (RHAG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 67714-1-Ig

    IHC staining of human placenta using 67714-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67714-1-Ig (RHAG antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

    Ready-to-use IHC kits available with RHAG antibody included
  • IHC staining of human placenta using 67714-1-Ig

    IHC staining of human placenta using 67714-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67714-1-Ig (RHAG antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

    Ready-to-use IHC kits available with RHAG antibody included
  • IF Staining of human placenta using 67714-1-Ig

    IF Staining of human placenta using 67714-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using RHAG antibody (67714-1-Ig, Clone: 1C1C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of human placenta using 67714-1-Ig

    IF Staining of human placenta using 67714-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using RHAG antibody (67714-1-Ig, Clone: 1C1C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

RHAG Monoclonal antibody

RHAG Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67714-1-Ig

产品说明书

CloneNo. 1C1C4

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IHC, IF-P, ELISA

别名

Ammonium transporter Rh type A, CD241, Rh associated glycoprotein, Rh family type A glycoprotein, Rh type A glycoprotein, RH2, Rh50, RH50A, Rh50GP, RHAG, SLC42A1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman placenta tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman placenta tissue
Planning an IHC experiment? We recommend our IHCeasy RHAG Ready-To-Use IHC Kit. RHAG primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67714-1-Ig targets RHAG in WB, IHC, IF-P, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 Human
免疫原 RHAG fusion protein Ag30130 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 Rh-associated glycoprotein
别名 Ammonium transporter Rh type A, CD241, Rh associated glycoprotein, Rh family type A glycoprotein, Rh type A glycoprotein, RH2, Rh50, RH50A, Rh50GP, RHAG, SLC42A1
计算分子量 409 aa, 44 kDa
观测分子量50-60 kDa
GenBank蛋白编号BC012605
基因名称 RHAG
Gene ID (NCBI) 6005
RRIDAB_2882904
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ02094
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RHAG antibody 67714-1-IgDownload protocol
IHC protocol for RHAG antibody 67714-1-IgDownload protocol
IF protocol for RHAG antibody 67714-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols