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  • Featured Product
  • KD/KO Validated

RHCG Polyclonal antibody

RHCG Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 11949-1-AP

Print datasheet

Synonyms

Tumor-related protein DRC2, RHGK, PDRC2, CDRC2, C15orf6

验证数据展示

  • WB analysis of HEK-293 using 11949-1-AP

    WB analysis of HEK-293 using 11949-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11949-1-AP (RHCG antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 11949-1-AP

    IHC staining of human kidney using 11949-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 11949-1-AP (RHCG antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 11949-1-AP

    IHC staining of human kidney using 11949-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 11949-1-AP (RHCG antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inHEK-293 cells, human kidney tissue
Positive IHC detected inhuman kidney tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11949-1-AP targets RHCG in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen RHCG fusion protein Ag2493 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Rh family, C glycoprotein
Synonyms Tumor-related protein DRC2, RHGK, PDRC2, CDRC2, C15orf6
Calculated Molecular Weight 479 aa, 53 kDa
Observed Molecular Weight 52-55 kDa
GenBank Accession NumberBC030965
Gene Symbol RHCG
Gene ID (NCBI) 51458
RRIDAB_2269482
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBD6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RHCG antibody 11949-1-APDownload protocol
IHC protocol for RHCG antibody 11949-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF,CoIP

Kidney Int

The ammonia transporter RhCG modulates urinary acidification by interacting with the vacuolar proton-ATPases in renal intercalated cells.

Authors - Soline Bourgeois
  • KO Validated
View Article
humanWB,IHC

Aging (Albany NY)

Rh type C-glycoprotein functions as a novel tumor suppressor gene by inhibiting tumorigenicity and metastasis in head and neck squamous cell carcinoma.

Authors - Wenguang Xu
View Article