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验证数据展示

  • WB analysis using 66733-1-Ig (same clone as 66733-1-PBS)

    WB analysis using 66733-1-Ig (same clone as 66733-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66733-1-Ig (RHOA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66733-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66733-1-Ig (same clone as 66733-1-PBS)

    IHC staining of human colon cancer using 66733-1-Ig (same clone as 66733-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66733-1-Ig (RHOA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66733-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RHOA antibody included
  • IHC staining of human colon cancer using 66733-1-Ig (same clone as 66733-1-PBS)

    IHC staining of human colon cancer using 66733-1-Ig (same clone as 66733-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66733-1-Ig (RHOA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66733-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RHOA antibody included
  • IF Staining of HeLa using 66733-1-Ig (same clone as 66733-1-PBS)

    IF Staining of HeLa using 66733-1-Ig (same clone as 66733-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66733-1-Ig (RHOA antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66733-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

RHOA Monoclonal antibody, PBS Only

RHOA Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66733-1-PBS

产品说明书

CloneNo. 1B3D7

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, pig, rat

应用

WB, IHC, IF/ICC, ELISA

别名

ARH12, ARHA, Rho cDNA clone 12, RHO12, RHOA, RHOH12, Transforming protein RhoA

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 
100 μg

¥5800/ 100 μg

浓度: 3732 μg/ml

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Planning an IHC experiment? We recommend our IHCeasy RHOA Ready-To-Use IHC Kit. RHOA primary antibody included.

产品信息

66733-1-PBS targets RHOA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, pig, rat
免疫原 RHOA fusion protein Ag1141 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ras homolog gene family, member A
别名 ARH12, ARHA, Rho cDNA clone 12, RHO12, RHOA, RHOH12, Transforming protein RhoA
计算分子量 22 kDa
观测分子量 22 kDa
GenBank蛋白编号BC005976
基因名称 RHOA
Gene ID (NCBI) 387
RRIDAB_2882083
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP61586
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RhoA is a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of RhoA is associated with tumor cell proliferation and metastasis. RhoA signalling is critical to many cellular processes including migration, mechanotransduction, and is often disrupted in carcinogenesis.