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  • Featured Product
  • KD/KO Validated

RHOA Monoclonal antibody, PBS Only

RHOA Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B3D7

Cat no : 66733-1-PBS

Print datasheet

Synonyms

ARH12, ARHA, Rho cDNA clone 12, RHO12, RHOA, RHOH12, Transforming protein RhoA

验证数据展示

  • WB analysis using 66733-1-Ig (same clone as 66733-1-PBS)

    WB analysis using 66733-1-Ig (same clone as 66733-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66733-1-Ig (RHOA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66733-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66733-1-Ig (same clone as 66733-1-PBS)

    IHC staining of human colon cancer using 66733-1-Ig (same clone as 66733-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66733-1-Ig (RHOA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66733-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RHOA antibody included
  • IHC staining of human colon cancer using 66733-1-Ig (same clone as 66733-1-PBS)

    IHC staining of human colon cancer using 66733-1-Ig (same clone as 66733-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66733-1-Ig (RHOA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66733-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RHOA antibody included
  • IF Staining of HeLa using 66733-1-Ig (same clone as 66733-1-PBS)

    IF Staining of HeLa using 66733-1-Ig (same clone as 66733-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66733-1-Ig (RHOA antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66733-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy RHOA Ready-To-Use IHC Kit. RHOA primary antibody included.

产品信息

66733-1-PBS targets RHOA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse, pig, rat
Immunogen RHOA fusion protein Ag1141 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ras homolog gene family, member A
Synonyms ARH12, ARHA, Rho cDNA clone 12, RHO12, RHOA, RHOH12, Transforming protein RhoA
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC005976
Gene Symbol RHOA
Gene ID (NCBI) 387
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP61586
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RhoA is a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of RhoA is associated with tumor cell proliferation and metastasis. RhoA signalling is critical to many cellular processes including migration, mechanotransduction, and is often disrupted in carcinogenesis.