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RHOBTB3 Polyclonal antibody

RHOBTB3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 13945-1-AP

Print datasheet

Synonyms

KIAA0878, RHOBTB3

验证数据展示

  • WB analysis using 13945-1-AP

    WB analysis using 13945-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13945-1-AP (RHOBTB3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 13945-1-AP

    IHC staining of mouse heart using 13945-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13945-1-AP (RHOBTB3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 13945-1-AP

    IHC staining of human placenta using 13945-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13945-1-AP (RHOBTB3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat adrenal gland using 13945-1-AP

    IHC staining of rat adrenal gland using 13945-1-AP

    Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 13945-1-AP (RHOBTB3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, K-562 cells
Positive IHC detected inmouse heart tissue, human placenta tissue, rat adrenal gland tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13945-1-AP targets RHOBTB3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen RHOBTB3 fusion protein Ag5025 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Rho-related BTB domain containing 3
Synonyms KIAA0878, RHOBTB3
Calculated Molecular Weight 611 aa, 69 kDa
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC041337
Gene Symbol RHOBTB3
Gene ID (NCBI) 22836
RRIDAB_2877992
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94955
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RHOBTB3 antibody 13945-1-APDownload protocol
IHC protocol for RHOBTB3 antibody 13945-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Theranostics

Circular RNA circRHOBTB3 represses metastasis by regulating the HuR-mediated mRNA stability of PTBP1 in colorectal cancer.

Authors - Jiaxin Chen
View Article
humanWB,IP

Cells

RhoBTB3 Functions as a Novel Regulator of Autophagy by Suppressing AMBRA1 Stability

Authors - Kyungho Kim
View Article