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RHOD Polyclonal antibody

RHOD Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11717-1-AP

Print datasheet

Synonyms

ARHD, Rho, Rho related protein HP1, RHOD, RHOHP1, RHOM

验证数据展示

  • IF Staining of Human Retina Whole Mount using 11717-1-AP

    IF Staining of Human Retina Whole Mount using 11717-1-AP

    Human Retina Whole Mount generated from human induced pluripotent stem cells (iPSCs) and fixed with 4% PFA. Stained for RHOD with 11717-1-AP at 1:400, ARR3 1:400 Nuclear stain DAPI (blue). Data generated by Alessandro Bellapianta at Johannes Kepler Universitat, Austria.

  • IHC staining of human lung cancer using 11717-1-AP

    IHC staining of human lung cancer using 11717-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11717-1-AP (RHOD Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 11717-1-AP

    IHC staining of human lung cancer using 11717-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11717-1-AP (RHOD Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inhuman lung cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHuman Retina Whole Mount

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11717-1-AP targets RHOD in IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF, IHC, ELISA Application Description
Tested Reactivity human
Immunogen RHOD fusion protein Ag2291 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ras homolog gene family, member D
Synonyms ARHD, Rho, Rho related protein HP1, RHOD, RHOHP1, RHOM
Calculated Molecular Weight 210 aa, 23 kDa
GenBank Accession NumberBC001338
Gene Symbol RHOD
Gene ID (NCBI) 29984
RRIDAB_2877789
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00212
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RhoD is a member of the Rho family of small GTPases, and it has been implicated in integrating actin reorganization and endosome motility. RhoD localizes to early endosomes and recycling endosomes, which indicates its important role in the regulation of endosome trafficking. It is involved in reorganization of the actin cytoskeleton, and it may coordinate membrane transport with the function of the cytoskeleton.

实验方案

Product Specific Protocols
IHC protocol for RHOD antibody 11717-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols