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RHOF Polyclonal antibody

RHOF Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 12290-1-AP

Print datasheet

Synonyms

ARHF, Rho family GTPase Rif, Rho in filopodia, RHOF, RIF

验证数据展示

  • WB analysis using 12290-1-AP

    WB analysis using 12290-1-AP

    Various cells were subjected to SDS PAGE followed by western blot with 12290-1-AP (RHOF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 12290-1-AP

    IHC staining of human colon cancer using 12290-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon cancer using 12290-1-AP

    IHC staining of human colon cancer using 12290-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 12290-1-AP (RHOF antibody) at dilution of 1:50 (under 40x lens).

  • IP experiment of COLO 320 using 12290-1-AP

    IP experiment of COLO 320 using 12290-1-AP

    IP result of anti-RHOF (IP:12290-1-AP, 4ug; Detection:12290-1-AP 1:300) with COLO 320 cells lysate 2400 ug.

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, human brain tissue, COLO 320 cells, mouse brain tissue, rat brain tissue
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman colon cancer tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

12290-1-AP targets RHOF in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen RHOF fusion protein Ag2943 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ras homolog gene family, member F (in filopodia)
Synonyms ARHF, Rho family GTPase Rif, Rho in filopodia, RHOF, RIF
Calculated Molecular Weight 211 aa, 24 kDa
Observed Molecular Weight24 kDa
GenBank Accession NumberBC018208
Gene Symbol RHOF
Gene ID (NCBI) 54509
RRIDAB_10644126
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HBH0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RHOF antibody 12290-1-APDownload protocol
IHC protocol for RHOF antibody 12290-1-APDownload protocol
IP protocol for RHOF antibody 12290-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Biol Chem

Rho family small GTPase Rif regulates Wnt5a-Ror1-Dvl2 signaling and promotes lung adenocarcinoma progression

Authors - Michiru Nishita
View Article
humanWB

Int J Mol Sci

An Integrated Multi-Omics Analysis Identifying Immune Subtypes of Pancreatic Cancer

Authors - Yongcheng Su
View Article