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验证数据展示

  • WB analysis using 21576-1-AP

    WB analysis using 21576-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21576-1-AP (RHOG antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

RHOG Polyclonal antibody

RHOG Polyclonal Antibody for WB, ELISA
Cat No. 21576-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ARHG, RHOG

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, MCF-7 cells, K-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21576-1-AP targets RHOG in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen RHOG fusion protein Ag16118 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ras homolog gene family, member G (rho G)
Synonyms ARHG, RHOG
Calculated Molecular Weight 191 aa, 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC104178
Gene Symbol RHOG
Gene ID (NCBI) 391
RRIDAB_2878887
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP84095
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RHOG, also named as ARHG, belongs to the small GTPase superfamily and Rho family. It is required for the formation of membrane ruffles during macropinocytosis. And it is required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, it is activated by SopB and SGEF, which induces cytoskeleton rearrangements and promotes bacterial entry. This antibody is specific to RHOG.

实验方案

Product Specific Protocols
WB protocol for RHOG antibody 21576-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols