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  • KD/KO Validated

MIRO2 Polyclonal antibody

MIRO2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IHC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 11237-1-AP

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Synonyms

ARHT2, C16orf39, hMiro 2, MIRO 2, miro2, Mitochondrial Rho GTPase 2, RASL, RHOT2



经过测试的应用

Positive WB detected inHEK-293T cells, human kidney tissue, mouse heart tissue, L02 cells, HepG2 cells, human testis tissue, human heart tissue, K-562 cells, Hela cells
Positive IP detected inL02 cells
Positive IHC detected inhuman colon tissue, human kidney tissue, human heart tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11237-1-AP targets MIRO2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP, CoIP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, monkey
Immunogen MIRO2 fusion protein Ag1752 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ras homolog gene family, member T2
Synonyms ARHT2, C16orf39, hMiro 2, MIRO 2, miro2, Mitochondrial Rho GTPase 2, RASL, RHOT2
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC014942
Gene Symbol RHOT2
Gene ID (NCBI) 89941
RRIDAB_2179539
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IXI1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RHOT2 (Ras homolog gene family, member T2), also known as ARHT2, RASL or MIRO-2 (mitochondrial Rho GTPase 2), is a 618 amino acid single-pass type IV membrane protein as a member of the Rho family of GTPases. It is localized to the outer mitochondrial membrane and plays a role in mitochondrial trafficking and fusion-fission dynamics. RHOT2 is ubiquitously expressed with highest levels found in pancreas, heart, kidney and skeletal muscle. MIRO2 is highly phosphorylated at basal conditions, as evidenced by an immunoreactive band that electrophoretically migrates 7 kDa higher (~75 kDa) compared to its predicted molecular weight (68 kDa) (PMID: 28556983).

实验方案

Product Specific Protocols
WB protocol for MIRO2 antibody 11237-1-APDownload protocol
IHC protocol for MIRO2 antibody 11237-1-APDownload protocol
IP protocol for MIRO2 antibody 11237-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
monkeyWB,IF

Nat Cell Biol

MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.

Authors - Tim König
  • KD Validated

Neuron

Brain-derived autophagosome profiling reveals the engulfment of nucleoid-enriched mitochondrial fragments by basal autophagy in neurons.

Authors - Juliet Goldsmith
WB

Sci Adv

Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.

Authors - Odetta Antico
humanIF

EMBO Rep

Parkin-mediated ubiquitylation redistributes MITOL/March5 from mitochondria to peroxisomes.

Authors - Fumika Koyano
humanWB, IP, IF

EMBO Rep

MICAL2PV suppresses the formation of tunneling nanotubes and modulates mitochondrial trafficking.

Authors - Fei Wang
humanWB, IF

Mol Ther Nucleic Acids

miR-351-5p/Miro2 axis contributes to hippocampal neural progenitor cell death via unbalanced mitochondrial fission.

Authors - Ha-Na Woo
  • KD Validated