• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

RHOT2 Monoclonal antibody

RHOT2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A3C2

Cat no : 68469-1-Ig

Print datasheet

Synonyms

ARHT2, C16orf39, hMiro 2, MIRO 2, Mitochondrial Rho GTPase 2, RASL, RHOT2

验证数据展示

  • WB analysis using 68469-1-Ig

    WB analysis using 68469-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68469-1-Ig (RHOT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68469-1-Ig

    WB analysis using 68469-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68469-1-Ig (RHOT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

经过测试的应用

Positive WB detected inU2OS cells, A549 cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HL-60 cells, THP-1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68469-1-Ig targets RHOT2 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen RHOT2 fusion protein Ag29690 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ras homolog gene family, member T2
Synonyms ARHT2, C16orf39, hMiro 2, MIRO 2, Mitochondrial Rho GTPase 2, RASL, RHOT2
Calculated Molecular Weight 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC014942
Gene Symbol RHOT2
Gene ID (NCBI) 89941
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8IXI1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RHOT2 (Mitochondrial Rho GTPase 2) is also named as MIRO-2, hMiro-2, ARHT2, C16orf39 and Ras homolog gene family member T2. RHOT2 belongs to the mitochondrial Rho GTPase family. RHOT2 gene encodes a member of Rho family of GTPase, which are localized to the outer membrane of mitochondria (PMID: 22078885).

实验方案

Product Specific Protocols
WB protocol for RHOT2 antibody 68469-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols