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  • Featured Product
  • KD/KO Validated

RHOV Polyclonal antibody

RHOV Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26620-1-AP

Print datasheet

Synonyms

ARHV, CDC42 like GTPase 2, Chp, GTP binding protein like 2, Rho GTPase like protein ARHV, RHOV, WRCH 2, WRCH2

验证数据展示

  • WB analysis of rat lung using 26620-1-AP

    WB analysis of rat lung using 26620-1-AP

    rat lung tissue were subjected to SDS PAGE followed by western blot with 26620-1-AP (RHOV Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 26620-1-AP

    IHC staining of human lung cancer using 26620-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26620-1-AP (RHOV Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 26620-1-AP

    IHC staining of human lung cancer using 26620-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 26620-1-AP (RHOV Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inrat lung tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26620-1-AP targets RHOV in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, rat
Cited Reactivityhuman
Immunogen RHOV fusion protein Ag24331 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ras homolog gene family, member V
Synonyms ARHV, CDC42 like GTPase 2, Chp, GTP binding protein like 2, Rho GTPase like protein ARHV, RHOV, WRCH 2, WRCH2
Calculated Molecular Weight 236 aa, 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC112945
Gene Symbol RHOV
Gene ID (NCBI) 171177
RRIDAB_2880576
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96L33
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RHOV antibody 26620-1-APDownload protocol
IHC protocol for RHOV antibody 26620-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Biol Sci

RHOV promotes lung adenocarcinoma cell growth and metastasis through JNK/c-Jun pathway.

Authors - Deyu Zhang
View Article
humanWB,IHC

Front Oncol

Overexpression of RhoV Promotes the Progression and EGFR-TKI Resistance of Lung Adenocarcinoma.

Authors - Hongjin Chen
  • KD Validated
View Article