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验证数据展示

  • WB analysis using 13460-1-AP

    WB analysis using 13460-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13460-1-AP (RHPN1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 13460-1-AP

    IP experiment of Jurkat using 13460-1-AP

    IP result of anti-RHPN1 (IP:13460-1-AP, 4ug; Detection:13460-1-AP 1:300) with Jurkat cells lysate 3400ug.

RHPN1 Polyclonal antibody

RHPN1 Polyclonal Antibody for WB, IP, ELISA
Cat No. 13460-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

GTP Rho binding protein 1, KIAA1929, ODF5, RHOPHILIN, Rhophilin 1, RHPN, RHPN1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA375 cells, human brain tissue, Jurkat cells, PC-3 cells, mouse brain tissue, rat brain tissue
Positive IP detected inJurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13460-1-AP targets RHPN1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RHPN1 fusion protein Ag3933 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 rhophilin, Rho GTPase binding protein 1
别名 GTP Rho binding protein 1, KIAA1929, ODF5, RHOPHILIN, Rhophilin 1, RHPN, RHPN1
计算分子量 670 aa, 74 kDa
观测分子量 74 kDa
GenBank蛋白编号BC025767
基因名称 RHPN1
Gene ID (NCBI) 114822
RRIDAB_2179682
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TCX5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RHPN1 antibody 13460-1-APDownload protocol
IP protocol for RHPN1 antibody 13460-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols