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RHPN2 Polyclonal antibody
RHPN2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | COLO 320 cells, HepG2 cells |
Positive IHC detected in | human colon tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12671-1-AP targets RHPN2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | RHPN2 fusion protein Ag3353 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | rhophilin, Rho GTPase binding protein 2 |
Synonyms | Rhophilin-2, Rhophilin 2, RhoBP, p76RBE, GTP-Rho-binding protein 2 |
Calculated Molecular Weight | 686 aa, 77 kDa |
Observed Molecular Weight | 77 kDa |
GenBank Accession Number | BC036447 |
Gene Symbol | RHPN2 |
Gene ID (NCBI) | 85415 |
RRID | AB_2877872 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q8IUC4 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for RHPN2 antibody 12671-1-AP | Download protocol |
IHC protocol for RHPN2 antibody 12671-1-AP | Download protocol |
IF protocol for RHPN2 antibody 12671-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Sci Signal RhoA inhibits neural differentiation in murine stem cells through multiple mechanisms. | ||
Cell Cycle P76RBE silencing inhibits ovarian cancer cell proliferation, migration, and invasion via suppressing the integrin β1/NF-κB pathway.
| ||
J Biochem Mol Toxicol Circ_0000284 upregulates RHPN2 to facilitate pancreatic cancer proliferation, metastasis, and angiogenesis through sponging miR-1179 |