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RI-1

RI-1 (RAD51 inhibitor 1) 是一种选择性 RAD51的抑制剂，IC50值为 5-30 μM，可以破坏人类细胞中的同源重组。它在半胱氨酸 319 处与 RAD51 蛋白表面共价结合，并通过直接结合到 RAD51 细丝中蛋白质亚基之间的界面的蛋白质表面来灭活 RAD51。

CAS号

415713-60-9

分子式

C14H11Cl3N2O3

主要靶点

DNA/RNA Synthesis

仅限科研使用

Cat No : CM06252

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Synonyms

RAD51 inhibitor 1|RI1|RI-1; 3-氯-1-(3,4-二氯苯基)-4-(4-吗啉基)-1H-吡咯-2,5-二酮

验证数据展示

  • CAS No.: 415713-60-9

    RI-1
    CAS#: 415713-60-9

产品信息

RI-1 (RAD51 inhibitor 1) is a RAD51 inhibitor (IC50: 5-30 μM).

CAS号 415713-60-9
分子式 C14H11Cl3N2O3
主要靶点 DNA/RNA Synthesis
主要通路 DNA损伤和修复|细胞周期
分子量 361.61
纯度 99.62%， 此纯度可做参考，具体纯度与批次有关系，可咨询客服
储存条件 Powder: -20°C for 3 years | In solvent: -80°C for 1 year
别名 RAD51 inhibitor 1|RI1|RI-1; 3-氯-1-(3,4-二氯苯基)-4-(4-吗啉基)-1H-吡咯-2,5-二酮

靶点活性

RAD51:<30 μM

体内活性

在HeLa细胞,MCF-7和U2OS三种癌细胞株中,RI-1具有单剂毒性（LD50：20-40 μM）。 通过直接和特异破坏HsRAD51和抑制RAD51,RI-1可抑制RAD51在ssDNA上形成丝的能力,从而使细胞对DNA损伤的敏感性增加。RI-1使G2期细胞的γ-H2AX灶减少,并使辐射6小时后未修复DSB的水平增加。

溶解度

DMSO:36.2 mg/mL (100 mM)

细胞实验

Cytotoxicity is determined by loss of colony-forming ability. Experiments are performed in triplicate. Crystal violet stained colonies are imaged with a CCD camera and counted using NIH Image software. Error bars denote standard error.(Only for Reference)

参考文献

1.Budke B, et al. Nucleic Acids Res. 2012, 40(15), 7347-7357.
2.Bee L, et al. PLoS One. 2013, 8(7), e69061.

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