- Featured Product
- KD/KO Validated
RIAM Polyclonal antibody
RIAM Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells |
Positive IP detected in | Jurkat cells |
Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 1 publications below |
WB | See 2 publications below |
IHC | See 1 publications below |
产品信息
13500-1-AP targets RIAM in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IP, IHC, ELISA Application Description |
Cited Applications | WB, IHC |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, chicken |
Immunogen | RIAM fusion protein Ag4327 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | amyloid beta (A4) precursor protein-binding, family B, member 1 interacting protein |
Synonyms | APBB1 interacting protein 1, APBB1IP, INAG1, PREL 1, PREL1, Proline rich EVH1 ligand 1, Proline rich protein 73, RARP 1, RARP1, RIAM |
Calculated Molecular Weight | 666 aa, 73 kDa |
Observed Molecular Weight | 100 kDa |
GenBank Accession Number | BC035636 |
Gene Symbol | RIAM |
Gene ID (NCBI) | 54518 |
RRID | AB_2057231 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q7Z5R6 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for RIAM antibody 13500-1-AP | Download protocol |
IHC protocol for RIAM antibody 13500-1-AP | Download protocol |
IP protocol for RIAM antibody 13500-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Microbiol Spectr Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization.
| ||
PLoS One HPK1 associates with SKAP-HOM to negatively regulate Rap1-mediated B-lymphocyte adhesion. | ||
Immunol Lett Non-cleavable talin rescues defect in the T-cell conjugation of T-cells deficient in the immune adaptor SKAP1. | ||
Oncol Lett Identification of prognostic biomarkers associated with metastasis and immune infiltration in osteosarcoma. |